首页|普通丝瓜PYL基因家族鉴定及其在果实发育中的表达分析

普通丝瓜PYL基因家族鉴定及其在果实发育中的表达分析

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[目的]探究普通丝瓜PYL基因在ABA处理下的表达模式,以期为深入研究其基因功能提供参考依据,也为分子抗性育种以及提高普通丝瓜品质和产量提供新思路.[方法]用生物信息学方法,对普通丝瓜PYL基因家族进行全基因组鉴定,染色体定位,以及基因结构、基因共线性、选择压力、进化亲缘关系和顺式作用元件分析,并通过qRT-PCR验证在不同浓度ABA下基因特征表达.[结果]在普通丝瓜全基因组中鉴定到11个LcPYL基因,CDS长度576～966 bp,编码蛋白均具有典型PYR_PYL_RCAR_like结构;共线性分析发现7对片段复制基因对,普通丝瓜PYL基因启动子含多个与植物激素相关的顺式作用元件,其成员的表达具有一定的组织特异性;普通丝瓜果实在不同浓度外源ABA处理下:2 mg/mL ABA处理LcPYLs表达无显著变化,4 mg/mL ABA处理LcPYL5、LcPYL7、LcPYL11显著上调表达,8 mg/mL ABA处理LcPYL1、LcPYL7开花当天显著表达.[结论]对普通丝瓜PYL家族成员进行了系统的鉴定与分析,明确其分子结构特征和表达模式,其中LcPYL1、LcPYL5、LcPYL7和LcPYL1 1可能是与内源ABA生物合成密切相关的关键基因.
Genome-wide identification of the PYL gene family in Luff a cylindrica and expression analysis in fruit development
[Objective]Expression of PYL genes in Luffa cylindrica under ABA treatment was studied to provide a reference for gene function and to provide foundation for molecular breeding to improve the quali-ty and yield of L.cylindrica.[Methods]Bioinformatics methods were used to analyze the distribution,chromosome location,gene structure,gene collinearity,selection pressure,evolutionary relationships,and cis-acting elements of the PYL gene family in L.cylindrica.Expression of the PYL gene family un-der different concentrations of ABA was verified by qRT-PCR.[Results]Eleven LcPYL genes were identi-fied in the genome of L.cylindrica.LcPYL genes had CDSs of 576-966 bp with typical PYRPYLRCAR_like structures.Collinear analysis identified seven repeating gene pairs that were predicted to have similar functions.LcPYL promoters contained cis-acting elements associated with plant hormones,and the ex-pression of its members was tissue-specific.Expression of LcPYLs in L.cylindrica fruits did not change at 2 mg/mL ABA.However,the expression of LcPYL5,LcPYL7,and LcPYLl1 was significantly in-creased at 4 mg/mL ABA,while LcPYL1 and LcPYL7 were expressed at the day of flowering at 8 mg/mL ABA.[Conclusion]Our findings suggest that LcPYL1,LcPYL5,LcPYL7,and LcPYL11 may be closely linked to endogenous ABA biosynthesis.

Luffa cylindricaPYL gene familyfruit developmentABA treatmentexpression analysis

王玉倩、彭丽娟、刘建汀、温庆放、钟凤林、朱海生

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福建农林大学园艺学院,福州 350002

福建省农业科学院作物研究所,福州 350013

福建省蔬菜遗传育种重点实验室,福州 350013

普通丝瓜 PYL基因家族 果实发育 外源ABA处理 表达分析

福建省属公益类科研院所基本科研专项福建省属公益类科研院所基本科研专项福建省自然科学基金项目福建省农业科学院科技创新团队项目国家大宗蔬菜产业体系福州综合试验站项目"5511"协同创新工程项目

2021R10310012022R10310072021J01492CXTD2021003-1CARS-23-G51XTCXGC2021003

2024

10.7606/j.issn.1000-4025.20230546

西北植物学报
西北农林科技大学,陕西省植物学会

西北植物学报

CSTPCD北大核心
影响因子：1.031
ISSN：1000-4025
年,卷(期)：2024.44(3)
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