普通丝瓜PYL基因家族鉴定及其在果实发育中的表达分析
Genome-wide identification of the PYL gene family in Luff a cylindrica and expression analysis in fruit development
王玉倩 1彭丽娟 1刘建汀 2温庆放 3钟凤林 1朱海生3
作者信息
- 1. 福建农林大学园艺学院,福州 350002
- 2. 福建农林大学园艺学院,福州 350002;福建省农业科学院作物研究所,福州 350013;福建省蔬菜遗传育种重点实验室,福州 350013
- 3. 福建省农业科学院作物研究所,福州 350013;福建省蔬菜遗传育种重点实验室,福州 350013
- 折叠
摘要
[目的]探究普通丝瓜PYL基因在ABA处理下的表达模式,以期为深入研究其基因功能提供参考依据,也为分子抗性育种以及提高普通丝瓜品质和产量提供新思路.[方法]用生物信息学方法,对普通丝瓜PYL基因家族进行全基因组鉴定,染色体定位,以及基因结构、基因共线性、选择压力、进化亲缘关系和顺式作用元件分析,并通过qRT-PCR验证在不同浓度ABA下基因特征表达.[结果]在普通丝瓜全基因组中鉴定到11个LcPYL基因,CDS长度576～966 bp,编码蛋白均具有典型PYR_PYL_RCAR_like结构;共线性分析发现7对片段复制基因对,普通丝瓜PYL基因启动子含多个与植物激素相关的顺式作用元件,其成员的表达具有一定的组织特异性;普通丝瓜果实在不同浓度外源ABA处理下:2 mg/mL ABA处理LcPYLs表达无显著变化,4 mg/mL ABA处理LcPYL5、LcPYL7、LcPYL11显著上调表达,8 mg/mL ABA处理LcPYL1、LcPYL7开花当天显著表达.[结论]对普通丝瓜PYL家族成员进行了系统的鉴定与分析,明确其分子结构特征和表达模式,其中LcPYL1、LcPYL5、LcPYL7和LcPYL1 1可能是与内源ABA生物合成密切相关的关键基因.
Abstract
[Objective]Expression of PYL genes in Luffa cylindrica under ABA treatment was studied to provide a reference for gene function and to provide foundation for molecular breeding to improve the quali-ty and yield of L.cylindrica.[Methods]Bioinformatics methods were used to analyze the distribution,chromosome location,gene structure,gene collinearity,selection pressure,evolutionary relationships,and cis-acting elements of the PYL gene family in L.cylindrica.Expression of the PYL gene family un-der different concentrations of ABA was verified by qRT-PCR.[Results]Eleven LcPYL genes were identi-fied in the genome of L.cylindrica.LcPYL genes had CDSs of 576-966 bp with typical PYRPYLRCAR_like structures.Collinear analysis identified seven repeating gene pairs that were predicted to have similar functions.LcPYL promoters contained cis-acting elements associated with plant hormones,and the ex-pression of its members was tissue-specific.Expression of LcPYLs in L.cylindrica fruits did not change at 2 mg/mL ABA.However,the expression of LcPYL5,LcPYL7,and LcPYLl1 was significantly in-creased at 4 mg/mL ABA,while LcPYL1 and LcPYL7 were expressed at the day of flowering at 8 mg/mL ABA.[Conclusion]Our findings suggest that LcPYL1,LcPYL5,LcPYL7,and LcPYL11 may be closely linked to endogenous ABA biosynthesis.
关键词
普通丝瓜/PYL基因家族/果实发育/外源ABA处理/表达分析Key words
Luffa cylindrica/PYL gene family/fruit development/ABA treatment/expression analysis引用本文复制引用
基金项目
福建省属公益类科研院所基本科研专项(2021R1031001)
福建省属公益类科研院所基本科研专项(2022R1031007)
福建省自然科学基金项目(2021J01492)
福建省农业科学院科技创新团队项目(CXTD2021003-1)
国家大宗蔬菜产业体系福州综合试验站项目(CARS-23-G51)
"5511"协同创新工程项目(XTCXGC2021003)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据