[Objective]As an important regulator involved in protein synthesis and gene expression,riboso-mal proteins are crucial for plant growth and development and stress response.In this study,a ribosomal protein family gene OsRPL36A was cloned from rice,and a preliminary study was performed for its bio-logical function,providing a basis for the study of OsRPL36A function.[Methods]Bioinformatics tech-niques were used to analysis gene structure,cis-regulatory elements,and evolution process of OsRPL36A.Meanwhile,the expression of OsRPL36A in different tissues and under diurnal rhythm,different hor-mones,and abiotic stresses were analyzed by quantitative real-time PCR(qRT-PCR).[Results](1)OsR-PL36A encoded 98 amino acids with a 297 bp coding region,which belonged to the ribosomal protein L36 supergene family,and was named as OsRPL36A.(2)The promoter region of OsRPL36A contained 3 rhythmic expression-related elements,10 light-responsive elements,14 hormone-responsive elements,and 27 environmental stress response elements.(3)The expression of OsRPL36A in leaves was relatively higher than that in other tissues and presented a typical rhythmic expression pattern.The expression of OsRPL36A was in-duced by IAA,high temperature,low temperature,osmotic stress,and so on.[Conclusion]OsRPL36A was highly expressed in leaves and presented a typical rhythmic expression pattern.OsRPL36A responded to IAA and might be involved in response to heat shock,low temperature,salt,and osmotic stresses.
NETL
NSTL
万方数据
维普
