摘要
[目的]核糖体蛋白(ribosomal protein,RP)是参与蛋白质合成及基因表达调控的一种重要因子,在植物生长发育和胁迫响应过程中具有重要的作用.研究在水稻中克隆了 1个核糖体蛋白家族基因OsRPL36A,并对其生物学功能进行初步分析,为后续OsRPL36A基因功能研究提供理论依据.[方法]用生物信息学技术分析OsR-PL36A基因结构、顺式作用元件和演化过程;用实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)技术分析OsRPL36A的组织表达特异性、节律表达模式、及其对不同激素和非生物胁迫的响应情况.[结果](1)OsR-PL36A的编码区全长为297 bp,共编码98个氨基酸,属于核糖体蛋白L36超基因家族.(2)OsRPL36A的启动子区包含3个节律表达相关元件、10个光响应元件、14个激素响应元件和27个环境胁迫响应元件.(3)OsRPL36A在叶片中的表达量相对高于其他组织;具有典型的节律表达模式;且受IAA、高温、低温和渗透胁迫等诱导表达.[结论]OsRPL36A在叶中高表达,具有典型节律表达模式,对IAA显著响应,可能参与热激、低温、盐害和渗透胁迫响应.
Abstract
[Objective]As an important regulator involved in protein synthesis and gene expression,riboso-mal proteins are crucial for plant growth and development and stress response.In this study,a ribosomal protein family gene OsRPL36A was cloned from rice,and a preliminary study was performed for its bio-logical function,providing a basis for the study of OsRPL36A function.[Methods]Bioinformatics tech-niques were used to analysis gene structure,cis-regulatory elements,and evolution process of OsRPL36A.Meanwhile,the expression of OsRPL36A in different tissues and under diurnal rhythm,different hor-mones,and abiotic stresses were analyzed by quantitative real-time PCR(qRT-PCR).[Results](1)OsR-PL36A encoded 98 amino acids with a 297 bp coding region,which belonged to the ribosomal protein L36 supergene family,and was named as OsRPL36A.(2)The promoter region of OsRPL36A contained 3 rhythmic expression-related elements,10 light-responsive elements,14 hormone-responsive elements,and 27 environmental stress response elements.(3)The expression of OsRPL36A in leaves was relatively higher than that in other tissues and presented a typical rhythmic expression pattern.The expression of OsRPL36A was in-duced by IAA,high temperature,low temperature,osmotic stress,and so on.[Conclusion]OsRPL36A was highly expressed in leaves and presented a typical rhythmic expression pattern.OsRPL36A responded to IAA and might be involved in response to heat shock,low temperature,salt,and osmotic stresses.
基金项目
广东省自然科学基金项目(2021A1515010517)
广东省自然科学基金项目(2022A1515010798)
大学生创新创业训练计划项目(S202211347059)
国家科技期刊平台
NSTL
维普
万方数据