[Objective]This study aims to identify the karyotypic characteristics and genetic relationships of 12 species of Oxalis from a cytologic view,in order to provide a reference for exploring chromosome diver-sity of Oxalis,identifying germplasm resources of Oxalis,and selecting parents for cross-breeding.[Methods]The chromosome morphology,number,and karyotype of 12 species of Oxalis plants were ob-served and analyzed by the root-tip compression method.[Results](1)Chromosome numbers were repor-ted for the first time for 7 species.Of the 5 reported species,O.fabaefolia and O.pes-caprae had chro-mosome numbers consistent with previous reports,while the remaining 3 differed from previous reports,of which 2n=26 was the rarely reported chromosome number in the genus Oxalis.A total of 7 chromo-some bases were found,of which x=13 was rarely reported,and ploidy ranged from 2x to 6x.Actual chromosome sizes ranged from 0.27 μm to 2.23 μm.The location of the attachment point was the meta-centric(m)and submetacentric(sm)chromosomes.There were 4 karyotype types and the karyotype asymmetry coefficients ranged from 56.31%to 65.40%.(2)O.fabaefolia was the most evolved while O.compressa was the most primitive.(3)According to the similarity of chromosomal karyotypes,the 12 species of Oxalis can be divided into 4 groups.[Conclusion]The 12 species of Oxalis have extensive kary-otypic diversity.Karyotypic classification is not in complete agreement with the morphological classifica-tions of previous reports.