茶树NAC转录因子基因CsNAC79和CsNAC9鉴定及其对非生物胁迫的响应
Identification of NAC transcription factor genes CsNAC79 and CsNAC9 in tea plant and their response to different abiotic stresses
孔洁玙 1杨妮 1罗微 1胡志航 1王雅慧 2庄静1
作者信息
- 1. 南京农业大学园艺学院,茶叶科学研究所,农业农村部华东地区园艺作物生物学与种质创制重点实验室,南京 210095
- 2. 南京农业大学作物遗传与种质创新利用全国重点实验室,南京 210095
- 折叠
摘要
[目的]鉴定茶树NAC转录因子基因CsNAC79和CsNAC9,并分析它们在干旱、盐、高温、低温、外施ABA和外施GA3胁迫下的表达模式,为进一步解析CsNAC79和CsNAC9转录因子的生物学功能提供参考.[方法]以茶树'龙井43'为材料,用RT-PCR扩增NAC转录因子家族基因CsNAC79和CsNAC9,并进行生物信息学分析;用qRT-PCR检测CsNAC79和CsNAC9在茶树6种非生物胁迫下的相对表达量.[结果](1)CsNAC79和CsNAC9分别编码409个和282个氨基酸,且分别在15-150氨基酸位点、11-134氨基酸位点含有NAC家族成员典型的NAM保守结构域.(2)CsNAC79蛋白与中华猕猴桃、柿、洋蓟亲缘关系较近,CsNAC9蛋白与桃、荔枝、榴莲亲缘关系较近;2个NAC转录因子均是亲水性蛋白,皆不含信号肽和跨膜结构;CsNAC79和CsNAC9的启动子区域都含有非生物胁迫响应元件;蛋白质空间结构分析显示,CsNAC79和CsNAC9蛋白主要是由不规则卷曲和a-螺旋组成.(3)表达分析表明:CsNAC79和CsNAC9基因在6种不同非生物胁迫下均上调表达.[结论]茶树CsNAC79和CsNAC9基因响应多种非生物胁迫.
Abstract
[Objective]The study aims to identify the NAC transcription factor genes CsNAC79 and Cs-NAC9 in tea plants and analyze their expression patterns under drought,salt,high temperature,and low temperature stresses as well as ABA and GA3 treatments in order to provide references for functional anal-ysis of CsNAC79 and CsNAC9.[Methods]CsNAC79 and CsNAC9 were amplified by RT-PCR from tea plant'Longjing 43'and analyzed by bioinformatics.The expression of CsNAC79 and CsNAC9 under abi-otic stresses in tea plants was analyzed by qRT-PCR.[Results](1)CsNAC79 and CsNAC9 encoded 409 and 282 amino acids,respectively,and contain the conserved NAM domain of the NAC family at 15-150 amino acids and 11-134 amino acids,respectively.(2)CsNAC79 protein was closely related to Actinidia chinensis,Diospyros kaki,and Cynara scolymus,while CsNAC9 protein was closely related to Amygda-lus persica,Litchi chinensis,and Durio zibethinus.Both of the NAC transcription factors were hydro-philic proteins without signal peptides and transmembrane structures.The promoter regions of CsNAC79 and CssNAC9 contained abiotic stress-responsive elements.Protein structure analysis showed that Cs-NAC79 and CsNAC9 proteins were mainly composed of random coils and a-helixes.(3)Expression analy-sis showed that CsNAC79 and CsNAC9 genes were up-regulated under different abiotic stresses.[Conclusion]The CsNAC79 and CsNAC9 genes in tea plants responded to various abiotic stresses.
关键词
茶树/NAC转录因子/ABA/GA3/非生物胁迫/表达分析Key words
tea plants/NAC transcription factor/ABA/GA3/abiotic stress/expression analysis引用本文复制引用
基金项目
国家自然科学基金项目(31870681)
江苏省政策引导类计划项目(SZ-LYG202126)
江苏高校优势学科建设项目(PAPD)()
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据