Micromorphological characteristics of leaf epidermis and its taxonomic significance of Phoebe plants in Hubei Province
[Objective]Most species of Phoebe are similar in morphology and the boundaries among species are blurring,making the identification and recognition of species difficult,particularly in the period with-out flowers and fruits.Micromorphological characteristics of leaves are valuable for the interspecific identi-fication of plants,but studies on Phoebe in this aspect are still scarce.[Methods]In this study,authors observed leaf morphology and micromorphology of eight species and one variant of Phoebe in Hubei Prov-ince using stereo microscope and light microscope.For all species,authors measured and analyzed both the quality and quantity properties of stomata and trichomes.The purpose was to provide the basis for the identification and classification of Phoebe.[Results]Four types of leaf shapes were characterized,inclu-ding large,medium,small,and irregular leaves of Phoebe plants.Trichomes were grouped into thin pu-bescent,thick pubescent,villous,and mixed pubescent and villous(villous trichomes are mainly found on leaf veins).The trichoid density of P.hunanensis(200 mm-2)differed significantly from P.bournei,P.chekiangensis,and P.sheareri(under 100 mm 2).Shapes of epidermis cells were polygon or irregular,and the anticlinal wall in epidermis cells was classified into straight-curved,sinuolate or undulated.The shape of stomata was wide oval or nearly round.Density of stomatal apparatus was the highest in P.sheareri and the lowest in P.chinensis and P.hunanensis(less than 500/mm2).In addition,there was a significant negative correlation between density and aspect ratio of stoma,as well as density and length of trichome.[Conclusion]The combination of morphological and micromorphological characteristics of leaves not only provided basis for identification and differentiation of the nine species but also provided references for interspecific identification of Phoebe.
NETL
NSTL
万方数据
维普
