Genome-wide identification and expression analysis of the SnRK2 gene family under drought stress in Saccharum spontaneum L.
[Objective]This study aims to explore the regulatory mechanism of SnRK2 family members un-der drought stress in Saccharum spontaneum L.,in order to provide candidate genes for cultivating drought resistant sugarcane varieties.[Methods]This study identified SnRK2 genes in S.spontaneum L.based on whole genome data,and conducted bioinformatics analysis and expression analysis under drought stress.[Results]11 SnRK2 members were identified and named as SsSnRK2.1 to SsSnRK2.11,which were unevenly distributed on 8 chromosomes.The amino acid residues of SsSnRK2s were ranged from 227 to 580,the molecular weights were from 25 683.53 kD to 64 695.8 kD,the isoelectric points were from 4.62 to 8.94,and all were hydrophilic proteins.The 11 SsSnRK2s could be divided into 3 subgroups,and the conserved motif sequences within the same subgroup were highly similar,with the number of exons ranging from 7 to 9.The promoters of SsSnRK2 members contained various hormone-related and abiotic stress responsive elements.SsSnRK2 members were expressed in a tissue-specific manner.All SsSnRK2 genes were able to respond to drought stress to various degrees.[Conclusion]The SsSnRK2 family plays a significant role in response to drought stress,providing a basis for the study of stress resistance in S.spontaneum L..
万方数据
维普
