Difference analysis of the potato roots metabolome under NaHCO3 stress
[Objective]The study aims to analyze the metabolomic difference in potato roots under NaHCO3 stress,in order to understand the metabolic mechanism of different potato varieties in response to NaHCO3 stress,and to provide theoretical basis for optimizing potato breeding,cultivation techniques,and production applications.[Methods]The root systems of potato'V7'and'KANG Nibeike'seedlings were selected as materials,and NaHCOs solution was used to simulate alkali stress with different concen-trations(CK,10,20,30,40,and 50 mmol/L).The root metabolites of these two potato varieties were analyzed by LC-MS and multidimensional statistics combined with non-targeted metabolomics detection.[Results](1)Under NaHCO3 stress,160 metabolites were up-regulated and 91 metabolites were down-regulated in'V7'roots,and 125 metabolites were up-regulated and 52 metabolites were down-regulated in'KANG Nibeike'roots.(2)10 differential metabolic pathways were selected for each of the two varieties,among which 4 differential metabolic pathways,namely biosynthesis of plant secondary metabolites,bio-synthesis of unsaturated fatty acids,synthesis of histidine and purine-derived alkaloids,and pyrimidine metabolism,were the key metabolic pathways in response to alkali stress in potato roots.(3)Differential metabolites,such as carbohydrates,amides,amines,oxy-containing organic compounds,alkaloids,phe-nolic acids,and somatogenin,etc.,were involved in the complex regulatory network in potato in response to NaHCO3 stress.[Conclusion]The key metabolites and metabolic pathways of potato roots in response to NaHCO3 stress are screened.There are differences in the metabolome of the alkaline tolerant cultivar'V7'and alkali sensitive cultivar'KANG Nibeike'.Also,there are differences between the metabolism of the same variety and CK under different levels of stress.The accumulation of amino acids and their deriva-tives,organic acids,and allantoin is an important feature of the'V7'root metabolism,which is more ac-tive and alkaline resistant than'KANG Nibeike'.
万方数据
