Identification and expression of the TIP subfamily in apple in response to drought stress
[Objective]This study aims to identify and analyze the members of the tonoplast intrinsic pro-tein(TIP)subfamily in apple,and to investigate the expression patterns under drought stress.It provides information for further research on utilization of drought resistance gene resources in apple.[Methods]The MdTIPs in apple genome was identified by bioinformatics methods.The physicochemical properties,gene structures,conserved motifs,cis-regulatory elements,and phylogenetic trees,etc.of the subfamily members were analyzed.The expression patterns of MdTIPs in different organs under drought stress were analyzed by qRT-PCR.[Results]A total of 13 MdTIP genes were identified in the apple genome,and most members were localized at the plasma membrane.Chromosome localization analysis suggested that all members were distributed on 10 chromosomes,with 1-3 members on each chromosome.Besides,the promoter regions of the genes contained response elements for hormonal and adversity stresses.qRT-PCR showed that MdTIP members were up-regulated in roots except MdTIP1;1,of which MdTIP1;3 and MdTIP1;4 were up-regulated 5.27 times and 5.69 times,respectively,compared with the control,suggesting that these two genes might be critical in response to drought stress.[Conclusion]Identification of MdTIP subfamily members is provided in this study.10 MdTIP members are differentially expressed in roots,stems,and leaves,and 12 members are highly expressed in roots.
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