[目的]通过组学技术研究持续喷水处理对芒果叶片产生的影响,揭示喷水处理影响"午休"的分子机制,进一步优化喷水处理条件,为达到最佳效果提供科学依据,并对提高芒果产量和品质以及响应国家减肥增效政策具有理论意义.[方法]以元江"台农一号"芒果为材料,对坐果期芒果在"午休"时段(12:30-14:10)喷水3次,每次喷水20 min,间隔20 min,以不喷水为对照,并以2组3个不同时期叶片进行Illumina Hi SeqTM 6000高通量转录组测序分析,用GO和KEGG数据库分析"午休"时段喷水和不喷水处理的差异表达基因(DEGs).用差异表达基因分析工具(DESeq)对基因表达进行差异分析,以|log2αFC|＞1,P＜0.05为筛选差异表达基因的条件.[结果]3个时期分别获得3 789,2 885,1 667个差异表达基因.GO分析表明差异基因与细胞组分分类中的膜、膜的固有成分、膜的组成成分等密切相关,KEGG分析差异表达基因主要富集在植物激素信号转导、植物MAPK信号通路、植物昼夜节律、氨基糖和核苷酸糖的代谢、类黄酮生物合成、光合生物碳固定、角质和亚硫酸盐及蜡的生物合成、卟啉和叶绿素的代谢、淀粉和蔗糖代谢以及甘油酯质代谢等途径.根据富集结果筛选8个差异表达基因,经实时荧光定量验证,基因表达趋势与转录组测序结果相符.[结论]CAO和POR基因表达延后,表明喷水处理可延长叶绿素的合成时间;FBP和SBP的基因表达略微降低,表明叶面喷水调控基因的表达量降至缓解芒果"午休"的最适范围内,有利于树体积累碳同化物,从而提高光合效率.
Transcriptome analysis of the effects of foliar water application on the nap time of mango leaves
[Objective]In order to reveal the molecular mechanism of photosynthetic midday-depression broken by water spring,the effects of continuous spraying on mango leaves were studied by omics technol-ogy.This study can provide basis for further optimizing water management to achieve the best conse-quences,and will have theoretical significance in enhancing mango yield and quality by aligning with the national policy objectives of reducing fertilizer usage and improving efficiency.[Methods]Mango cultivar"Tainong No.1"in Yuanjiang was used as material.The mango in the fruiting stage was sprayed by water for three times during the midday depression(12:30-14:10),20 minutes each time,with no water spra-ying as the control.The leaves were collected for transcriptome sequencing analysis.GO and KEGG data-bases were used to analyze the differentially expressed genes(DEGs).Analysis of gene expression was conducted by using DESeq,with|log2αFC|＞1 and P＜0.05 for DEG screening.[Results]3 789,2 885,and 1 667 differentially expressed genes were obtained from three development stages,respectively.GO analysis found that the DEGs were highly correlated with the membrane,intrinsic components of the membrane,components of the membrane and other items in the classification of cell components.KEGG analysis showed that the DEGs were mainly enriched in the pathways of plant hormone signal transduc-tion,MAPK signal pathway,circadian rhythm,metabolism of amino sugar and nucleotide sugar,fla-vonoid biosynthesis,photosynthetic biological carbon fixation,biosynthesis of cutin,sulfite,and wax,metabolism of porphyrin and chlorophyll,starch and sucrose metabolism,and glyceride metabolism.Ac-cording to the enrichment results,eight differentially expressed genes were screened.With qRT-PCR veri-fication,the gene expression trend was generally consistent with the results of RNA-seq.[Conclusion]The delayed expression of CAO and POR genes suggests that water spraying can prolong chlorophyll syn-thesis time.Expression of genes encoding FBP and SBP is slightly decreased,suggesting that water spray may regulate gene expression and ease photosynthetic"midday-depression",which is beneficial to carbon assimilation and photosynthetic efficiency.
mangophotosynthetic midday-depressiontranscriptomebiosynthesis of chlorophyllcar-bon fixation in photosynthetic organisms
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