摘要
[目的]探究不同生境中的牡丹对平原地区环境的形态适应特征,为牡丹的保育研究及筛选优质种质资源提供参考.[方法]以中原牡丹('凤丹'、'洛阳红')和野生牡丹(紫牡丹、狭叶牡丹、大花黄牡丹、西藏黄牡丹、滇牡丹、卵叶牡丹)为试验材料,采用石蜡切片技术观察叶片结构并分析相关指标.[结果](1)中原牡丹与野生牡丹叶表皮形态表现出不同程度的差异性,叶片为异面叶,上、下表皮细胞形状不规则,气孔为下生型.(2)大花黄牡丹的叶片与角质层较薄、栅栏组织不发达、细胞间隙较大且气孔密度大;滇牡丹的叶片、栅栏组织和海绵组织的厚度最大.(3)气孔密度与上表皮厚度、叶绿素b与叶绿素a/b、总叶绿素与叶绿素a/b呈极显著负相关关系(P＜0.01);气孔指数与栅栏组织厚度、栅栏组织厚度与叶片厚度、叶绿素b与总叶绿素含量呈极显著正相关关系.[结论]野生牡丹中除大花黄牡丹外其他种质均对平原生境有较强的适应性,大花黄牡丹的湿生结构可作为不能在平原地区栽培的主要原因.
Abstract
[Objective]We aim to investigate the morphological adaptation characteristics of peony in differ-ent habitats to the environment in the plain areas,and to provide references for peony conservation re-search and screening of high-quality germplasm resources.[Methods]The Central Plain peonies(Paeonia ostii'Fengdan'and P.'Luoyanghong')and wild peonies(P.delavayi Franch,P.potanini,P.lud-lowii,P.lutea(Tibet),P.lutea and P.qiui)were used as materials,and paraffin section technique was used to observe the leaf structure and analyze the related indexes.[Results](1)The leaf epidermal morphology of the Central Plain peonies and wild peonies showed varying degrees of variation.The leaves were heterofacial,with the upper and lower epidermal cells were irregularly shaped,and the stomata was uniformly distributed in the lower epidermis.(2)The leaves and cuticles of P.ludlowii were thin.The palisade tissue was underdeveloped.The intercellular space was large and the stomatal density was high.The thickness of leaves,palisade tissue,and sponge tissue of P.lutea was the largest.(3)Stomatal den-sity and epidermal thickness,chlorophyll b and chlorophyll a/b,total chlorophyll and chlorophyll a/b were significantly negatively correlated(P＜0.01).Stomatal index is positively correlated with palisade tissue thickness,palisade tissue thickness and leaf thickness,chlorophyll b and total chlorophyll.[Conclusion]The wild peony germplasms except P.ludlowii have strong adaptability to the plain habi-tats,and the wet structure of P.ludlowii is the main reason why it cannot be cultivated in the plain area.
基金项目
国家重点研发计划支持课题项目(2018YFD1000401)
中原领军人才项目(ZYYCYU202012129)
河南省科技攻关项目(232102111107)
河南省重点研发专项(231111110600)
国家科技期刊平台
NSTL
万方数据