Cloning and disease resistance function analysis of the phytosulfokine gene StPSK4 from potato
[Objective]The study aims to provide a theoretical basis for potato disease resistance breeding by investigating the characteristics of the potato defensin gene StPSK4 and conducting a functional analy-sis of its role in potato disease resistance.[Methods]The authors utilized bioinformatics method for a sys-tematic analysis of StPSK4,and conduct transcriptome sequencing analysis to examine the tissue-specific expression pattern of StPSK4 under biotic and abiotic stress,assessing the plant innate immune response to Pseudomonas syringae in StPSK4 overexpressing plants.[Results]The full length CDS of StPSK4 was 457 bp,encoding 100 amino acids.StPSK4 contained a signal peptide,with its tertiary structure mainly composed of α-helices and random coils.The C-terminus of PSK4 contains the defensin sequence DYIYTQ,and the similarity between StPSK4 and Solanaceae crops was above 80％.StPSK4 was highly expressed in potato sprouts and petioles,and showed strong response to biotic and abiotic stress such as high temperature,salt,P.syringae,and Phytophthora infestans.Overexpression of StPSK4 sup-pressed ROS burst in potato and defensed marker gene expression,and enhanced resistance to P.syrin-gae.[Conclusion]StPSK4 responds to various stress conditions such as high temperature and bacterial wilt infestans.Overexpression of StPSK4 inhibits ROS burst,defense marker gene expression,and re-sistance to bacterial wilt in potato,confirming the suppressive role of StPSK4 in potato disease resistance.
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