首页|甘蓝型油菜种子硫代葡萄糖苷含量的QTL定位及候选基因分析

甘蓝型油菜种子硫代葡萄糖苷含量的QTL定位及候选基因分析

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  • NSTL
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[目的]解析甘蓝型油菜种子硫代葡萄糖苷性状的重要遗传位点及候选基因,为甘蓝型油菜硫苷基因克隆和品种品质改良奠定基础.[方法]以甘蓝型油菜KN DH群体种子为材料,对在4个种植环境的种子硫苷含量进行QTL定位和候选基因分析.[结果](1)甘蓝型油菜种子硫苷含量变异系数较高且较为稳定,服从数量性状的遗传特点.(2)7 个一致性 QTL(cqGC.A9-5、cqGC.A9-7、cqGC.A9-9、cqGC.C2-9、cqGC.C2-10、cqGC.C9-5 和 cqGC.C9-6)为环境稳定表达 QTL,包括 3 个主效 QTLcqGC.A9-5、cqGC.C2-10 和 cqGC.C9-5).(3)在主效 QTL cqGC.A9-5 和 cqGC.C9-5 鉴定到 3 个候选基因(BnaA09g05480D、BnaC09g05620D 和 BnaC09g05810D),主要涉及硫苷生物合成途径中吲哚-3-乙醛肟、3-烷基-苹果酸的合成及硫苷的转运与分配.[结论]甘蓝型油菜种子硫苷含量为数量性状.3个甘蓝型油菜硫苷含量主效QTL被鉴定到,其置信区间候选基因的拟南芥同源基因参与硫苷合成途径中间产物合成及促进硫苷的转运与分配.
QTL mapping and candidate gene identification of seed glucosinolate content in Brassica napus
[Objective]The study aims to discover the genetic loci and candidate genes of seed glucosinolate content in Brassica napus,and lay a foundation for the cloning of genes involved in glucosinolate metabo-lism and improving the breeding of B.napus.[Methods]Using KN DH populations from four different environments as materials,the QTL mapping of seed glucosinolate content and the candidate genes were analyzed.[Results](1)The variation coefficient of seed glucosinolate content in B.napus was high and stable,which followed the genetic characteristics of quantitative traits.(2)Seven consistent QTLs,inclu-ding cqGC.A9-5,cqGC.A9-7,cqGC.A9-9,cqGC.C2-9,cqGC.C2-10,cqGC.C9-5 and cqGC.C9-6,were environmentally stable QTLs,of which cqGC.A9-5,cqGC.C2-10,and cqGC.C9-5 were major QTLs.(3)Three candidate genes,including BnaA09g05480D,BnaC09g05620D,and BnaC09g05810D,were identi-fied in the interval of the major QTL cqGC.A9-5 and cqGC.C9-5.Based on their annotation,these candi-date genes were involved in the biosynthetic pathway of glucosinolates(the biosynthesis of IAOx and 3-al-kyl malic acid)and transport and distribution of glucosinolates.[Conclusion]Seed glucosinolate content in B.napus was quantitative trait.Three major QTLs for seed glucosinolate content were identified,which might be involved in the synthesis of intermediate products and the transport and distribution of glucosino-lates.

Brassica napus L.seed glucosinolate contentQTL mappingcandidate gene

赵卫国、塔娜、王灏

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安康学院现代农业与生物科技学院,陕西安康 725000

陕西省杂交油菜研究中心,陕西杨凌 712100

甘蓝型油菜 种子硫苷含量 QTL定位 候选基因

陕西省高等学校青年创新团队项目陕西省大学生创新创业训练计划陕西省重点研发计划陕西省茶产业协同创新项目陕西省茶产业协同创新项目安康学院高层次人才引进项目

2023AYTD01S2023113970102020ZDLNY04-0123JY00123JY0032021AYQDZR12

2024

10.7606/j.issn.1000-4025.20240120

西北植物学报
西北农林科技大学,陕西省植物学会

西北植物学报

CSTPCD北大核心
影响因子：1.031
ISSN：1000-4025
年,卷(期)：2024.44(8)