Regulatory mechanism of exogenous myo-inositol improving high temperature stress tolerance of tomato seedlings
[Objective]Studing the effect of exogenous myo-inositol on the physiological and biochemical in-dexes of tomato seedlings and the molecular mechanism of tolerance to high temperature stress will provide a theoretical basis for the improvement of heat resistance of tomato.[Methods]Tomato variety'NRP20-7'was used as the test material,and water and myo-inositol were sprayed separately at normal tempera-ture and high temperature,respectively.The phenotypic changes under treatments were analyzed by hy-perspectral imaging,and the physiological indexes of each treatment group were analyzed.The expression of heat responsive genes,antioxidant,and ABA-related genes were analyzed by qRT-PCR.[Results]20 mmol/L myo-inositol treatment could effectively alleviate the damage of high temperature stress on tomato seedlings,reduce the heat damage index of tomato leaves,increase the maximum photochemical efficiency(Fv/Fm),and significantly increased the content of photosynthetic pigments.Exogenous myo-inositol in-creased proline and soluble sugar contents and peroxidase activity,and decreased malondialdehyde,hydro-gen peroxide,and superoxide anion contents in tomato seedlings at high temperature.Exogenous myo-ino-sitol increased the expression of heat response genes,antioxidant-related genes,ascorbic acid synthesis genes,and decreased the expression of heat shock protein genes.In addition,exogenous myo-inositol re-sponded to high temperature stress by regulating abscisic acid synthesis and signaling pathways to regulate the level of ABA in tomato seedlings.[Conclusion]Exogenous myo-inositol enhances tomato tolerance to high temperature stress by regulating complex biological processes in plants.
tomatohigh temperature stressmyo-inositolstress resistancegene expression
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