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蓖麻ZF-HD基因家族鉴定及表达模式分析

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[目的]对蓖麻ZF-HD家族基因进行鉴定,并对其成员进行序列分析、系统进化分析、启动子及表达模式分析,为进一步研究蓖麻中ZF-HD基因的功能提供参考.[方法]以蓖麻全基因组数据为基础,从中鉴定出ZF-HD 基因,并进行生物信息学分析和非生物胁迫下的表达分析.[结果]从蓖麻中共鉴定到13个ZF-HD基因家族成员,该基因家族成员不均匀分布在Chr1-Chr10上,13个RcZF-HD蛋白均为亲水性不稳定蛋白,亚细胞定位于细胞核.RcZF-HD基因家族聚类为3个亚簇,且每簇基因数不同,基因结构具有簇内保守性和簇间多样性.Rc-ZF-HD 基因家族成员具有大量组织特异性元件、应激响应元件和激素响应元件.ZF-HD基因广泛响应非生物胁迫,且表达具有组织特异性.[结论]在蓖麻中一共鉴定出13个ZF-HD基因.研究发现蓖麻ZF-HD基因家族成员在应对非生物胁迫时起重要作用,能够为蓖麻的抗逆性研究提供一定的理论依据.
Identification and expression analysis of the ZF-HD gene family in castor
[Objective]The ZF-HD family genes of castor were identified and analyzed by sequence analy-sis,phylogenetic analysis,promoter and expression pattern analysis,to provide a reference for further study of the function of ZF-HD genes in castor.[Methods]Based on the whole genome data of castor,ZF-HD genes were identified,and bioinformatics and expression analysis under abiotic stress were carried out.[Results]A total of 13 ZF-HD genes were identified from castor,which were unevenly distributed on Chr1-Chr10.All 13 RcZF-HD proteins were hydrophilic unstable proteins,and were localized in the nucle-us.The RcZF-HD genes were clustered into three subgroups,and the number of genes in each group was different.The gene structure was conserved within the group but diversified between groups.RcZF-HD genes had a large number of tissue-specific elements,stress response elements,and hormone response ele-ments.The ZF-HD genes were widely responsive to abiotic stresses and their expression was tissue-spe-cific.[Conclusion]A total of 13 ZF-HD genes were identified in castor.It is known that members of the castor ZF-HD gene family play an important role in responding to abiotic stress,this research provides theoretical basis for the study of castor's stress resistance.

castorRcZF-HD gene familybioinformaticsabiotic stressexpression mode

徐兴源、黄凤兰、张春兰、朱梦洋、崔志宇、刘鹏

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内蒙古民族大学农学院,内蒙古通辽 028000

内蒙古民族大学生命科学与食品学院,内蒙古通辽 028000

蓖麻育种国家民委重点实验室,内蒙古通辽 028000

内蒙古自治区高校蓖麻产业工程技术研究中心,内蒙古通辽 028000

内蒙古自治区蓖麻育种与综合利用重点实验室,内蒙古通辽 028000

蓖麻产业技术创新内蒙古自治区工程研究中心,内蒙古通辽 028000

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蓖麻 RcZF-HD基因家族 生物信息学 非生物胁迫 表达模式

2024

西北植物学报
西北农林科技大学,陕西省植物学会

西北植物学报

CSTPCD北大核心
影响因子:1.031
ISSN:1000-4025
年,卷(期):2024.44(12)