Characterization and phylogenetic analysis of the chloroplast genome of endangered Impatiens chishuiensis
[Objective]The chloroplast genome structure and phylogenetic location of Impatiens chishuien-sis were studied in order to provide scientific basis for the conservation of plant germplasm resources,ge-netic diversity,and phylogeny of I.chishuiensis.[Methods]Based on the chloroplast genome sequence of I.chishuiensis,an endemic species in China,the assembly,annotation,gene characterization,sequence repetition and phylogenetic analysis of chloroplast genome were performed by bioinformatic softwares.[Results](1)The chloroplast genome of I.chishuiensis has a typical tetrameric structure with a total GC content of 37％and a length of 152 892 bp.It encodes a total of 120 genes,including 95 protein-coding genes,8 rRNA genes,and 17 tRNA genes.(2)A total of 87 SSR sequences was detected.20 327 codons were detected,with leucine(Leu)being the most abundant and tryptophan(Trp)being the least.(3)Im-patiens was divided into two subgenera,including subg.Clavicarpa and subg.Impatiens.I.chishuiensis is a species of subg.Clavicarpa and is the closest with I.alpicola.[Conclusion]I.chishuiensis has a typical tetrad structure,and the SSR sequence is mainly formed by A/T single base.Phylogenetic analyses place it in the subg.Clavicarpa.
Impatiens chishuiensischloroplast genomesequence structure and characteristic analysisphylogeny
万方数据
维普
