Determination of Selenium in Tartary Buckwheat by Wet Digestion and Fluorescence Spectrophotometry
[Objective]The selenium content of 87 tartary buckwheat seeds from different sources was determined by wet digestion and fluorescence spectrophotometry in order to identify the resources of tartary buckwheat with high selenium content and further select and cultivate the seed rich in selenium.[Method]The selenium content of 87 tartary buck-wheat seed resources was determined by wet digestion-fluorescence spectrophotometry with the recovery rate and diges-tion time as the evaluation indexes.[Result]At four digestion temperatures(200,220,240 and 260 ℃),the recovery of selenium was 87.2%-102.4%.The higher the digestion temperature is,the shorter the digestion time;whereas the higher the temperature is,the easier the sample is to explode and evaporate locally,which reduces the recovery rate.Consider-ing all factors,the digestion temperature is selected at 240℃.The standard deviation of selenium content in 10 tartary buckwheat seed samples was 0.45 by wet digestion and fluorescence spectrophotometry.The selenium content of 87 tar-tary buckwheat seeds varied from 0.010 9 mg/kg to 0.179 0 mg/kg,with an average of 0.056 0 mg/kg.[Conclusion]42 va-rieties of tartary buckwheat with higher content(>0.050 0 mg/kg)were selected,which provided a theoretical basis for the study of selenium-rich tartary buckwheat products.
NETL
NSTL
万方数据
维普
