Isolation and Identification of Antagonistic Strain T9 Against Pomegranate Fusarium Wilt
[Objective]Pomegranate wilt disease is a soil borne disease caused by the sweet potato black rot Ceratocystis fimbriata Ellis.et Halsted,which causes devastating damage to pomegranates.The use of antagonistic microorganisms to inhibit the proliferation of pathogens and reduce their harm has become an effective way for green prevention and control of pomegranate wilt disease.This paper screened microorganisms that have antagonistic effects on the pathogen of pome-granate wilt,studied their inhibitory effects,and provided bacterial resources for the biological prevention and control of pomegranate wilt disease.[Method]Fungi isolated from the rhizospheric soil of pomegranate were screened based on spore production,growth rate,antagonistic coefficient,and plate confrontation results.Fungi with better control effects were identified using morphology and molecular systematics,and their control effects on pomegranate wilt disease were evaluated through pot experiments.[Result]The strain T9,which can effectively inhibit the growth of pomegranate wilt fungus hyphae,has been identified as African Trichoderma harzianum,and the highest control effect of potted plants can reach 71.35%.[Conclusion]This strain exhibits significant biological control effects on pomegranate wilt disease and has potential application values.
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