首页|水稻散生突变体sg518的鉴定及基因定位

水稻散生突变体sg518的鉴定及基因定位

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[目的]分蘖角作为水稻株型的重要组成部分,其遗传基础复杂,需鉴定更多与分蘖角相关的突变体和基因以进一步解析水稻分蘖角的分子调控机制.[方法]通过对贵州地方粳稻品种桥港珍珠米进行 60Co辐射诱变后成功获得一份水稻散生突变体,暂命名为sg518(spreading-grown mutant 518),并对其进行表型鉴定、遗传分析、基因定位和候选基因测序验证.[结果](1)田间性状显示,与野生型相比,突变体sg518表现出分蘖角显著增大的散生表型,并伴有一次枝梗数增加、每穗实粒数增加、籽粒变小和千粒重降低;(2)遗传分析表明,该突变体的散生性状受一对隐性核基因控制;(3)精细定位结果显示,目标基因定位于11号染色体长臂RM287与J1标记之间的88.7 kb内;(4)MutMap分析表明,在此区间有1个SNP频率为1的InDel突变且位于LAZY1基因上;(5)对目标基因的PCR扩增与测序结果表明,突变体的LAZY1基因在第3外显子上有1处1 bp的碱基缺失,导致蛋白翻译提前终止,由此推断LAZY1基因为突变体sg518的候选基因.[结论]sg518为LAZY1基因的一个新等位突变体,可进一步为研究该基因功能及水稻分蘖角的调控机制提供理论基础.
Identification and Gene Mapping of a Spreading-Grown Rice Mutant sg518
[Objective]As an important component of rice plant architecture,tiller angle has a complex genetic founda-tion.More mutants and genes associated with tiller angle need to be identified to further elucidate the molecular mecha-nisms of tiller angle in rice.[Method]A rice spreading-grown mutant,tentatively named sg518(spreading-grown mu-tant 518),was obtained through 60Co radiation mutagenesis to the local japonica rice variety Qiao-Gang Pearl Rice in Guizhou Province.Phenotypic identification,genetic analysis,gene mapping and candidate gene sequencing were con-ducted on this mutant.[Result](1)Field traits showed that the mutant sg518 displayed a spreading-grown phenotype with a significantly increased tiller angle,along with a higher number of primary branches,increased grains per panicle,reduced grain size,and lower 1 000 grain weight compared to the wild type.(2)Genetic analysis indicated that the spreading-grown phenotype in the sg518 mutant was controlled by a single recessive nuclear gene.(3)Fine mapping re-vealed that the target gene was situated in an 88.7 kb region between RM287 and J1 on the long arm of chromosome 11.(4)MutMap analysis indicated a single InDel mutation with a SNP index of 1 was located on LAZY1 gene within this re-gion.(5)PCR amplification and sequencing verified a 1 bp deletion in exon 3 of LAZY1 gene in the sg518 mutant,lead-ing to premature termination of protein translation.Therefore,it was inferred that LAZY1 gene was the candidate gene for the sg518 mutant.[Conclusion]sg518 is a novel allelic mutant of LAZY1 gene,providing a theoretical foundation for fur-ther exploration into the function of this gene and the regulatory mechanisms of tiller angle in rice.

ricea spreading-grown mutant sg518phenotypic identificationgene mappingLAZY1 gene

王洋、张文锋、杨杰、沙忠玉、韩文利、陈能刚、郭欣

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西昌学院 农业科学学院,四川 西昌 615013

西昌学院 攀西特色作物研究与利用四川省重点实验室,四川 西昌 615013

四川农业大学西南作物基因资源发掘与利用国家重点实验室,四川 成都 611130

云南农业大学农学与分子技术学院,云南 昆明 650000

贵州省农业科学院农作物品种资源研究所,贵州 贵阳 550006

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水稻 散生突变体sg518 表型鉴定 基因定位 LAZY1基因

国家自然科学基金项目西南作物基因资源发掘与利用国家重点实验室开放课题项目凉山州科技计划项目攀西特色作物研究与利用四川省重点实验室资助课题西昌市科技计划项目

32260502SKL-KF20222121ZDYF0020XNFZ2116kjcx-2021-02

2024

10.16104/j.issn.1673-1891.2024.02.001

西昌学院学报(自然科学版)
西昌学院

西昌学院学报(自然科学版)

影响因子：0.307
ISSN：1673-1891
年,卷(期)：2024.38(2)