Phylogenetic Analysis of the ITS Gene of Cordyceps sinensis and the COI Gene of Its Host Hepiaua larva in the Main Production Areas of China
[Objective]To analyse the ITS gene sequence of Cordyceps sinensis and the COI gene sequence of its host Hepi-aua larva so as to study their genetic differentiation and variation degree.[Method]Using phylogenetic analysis,the phy-logeny trees were constructed based on the analyses of ITS gene and COI gene,and haplotype diversity(Hd)and nucleo-tide diversity(Pi)of the sequences were calculated.[Result]The ITS gene sequences of Cordyceps sinensis could be di-vided into 17 haplotypes,with Hd=0.875 and Pi=0.001 19.The COI gene sequences of Hepiaua larva could be divided into 18 haplotypes,with Hd=1.000 and Pi=0.000 34.Using the gene replacement rate,it was estimated that Cordyceps si-nensis dated back to at least 58.3 million years ago while the Hepiaua larva originated from about 17.5 million years ago.[Conclusion]The genetic differences between Cordyceps sinensis and its host Hepiaua larva are both unrelated to the lati-tude where they are located and their genetic distances show no correlation with their geographical distances.The Cordy-ceps sinensis and its host Hepiaua larva possess rich genetic and differentiation diversities in the main production areas of China,which may be one of the origins of them.The differentiation time of Cordyceps sinensis was significantly earlier than that of Hepiaua larva,and it was speculated that Cordyceps sinensis might have existed in an indigenous way or had other hosts before parasitizing Hepiaua larva.After parasitizing Hepiaua larva,a co-evolutionary relationship was formed between them,which promoted the diversity and differentiation of the Hepiaua larva.
万方数据
维普
