Effects of Two Chemicals on the Differentiation of Rice Callus and Identification of Herbicide Resistance of the Differentiated Seedlings
[Objective]Rice callus were screened by chemical mutagenesis and herbicide,and new rice germplasms re-sources with herbicide resistance and new loci of acetolactate synthase(ALS)gene were identified.[Method]In this study,the effects of sodium azide(NaN3)and imazamox on the differentiation of rice callus were analyzed.And the her-bicide resistance identification and molecular identification were performed the differentiated seedlings.[Result]The NaN3 treatment could increase the differentiation rate when the medium was added NaN3.The highest differentiation rate was 33.30%on the treatment of 0.005 g/L imazamox solution and 2 mmol/L NaN3 solution.The differentiated seedlings were screened using different concentrations of imazamox.The differentiated seedlings were sprayed with 1 times(3.16 g/L)and 2 times(6.32 g/L)concentration of imazamox,and their survival rate reached 20%under the treatment of twice the recommended concentration.It proved that these survived seedlings had resistance to imazamox.The resistant seed-lings were sequenced by second generation sequencing.It showed that the base C was mutated to A at position 199 of the ALS gene,resulting in a change from proline to threonine at the position.[Conclusion]New rice germplasm resistant to imazamox were obtained.The mutation of Pro67Thr is a new resistant locus to imazamox.Our research provides germ-plasm resources and genetic resources for the study of rice herbicide-resistant mutagenesis breeding.
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维普
