摘要
采用循环伏安法(cyclic voltammetry,CV)将瓜氨酸(citrulline,Cit)聚合在玻碳电极上,得到了聚瓜氨酸修饰电极(polycitrulline modified glassy carbon electrode,PCit/GCE),并对尿酸(uric acid,UA)在PCit/GCE上的电化学行为进行研究,发现UA在PCit/GCE上的氧化峰电流与裸玻碳电极相比明显增大,灵敏度明显提高.UA的氧化峰电流与其浓度呈正比,线性范围为 2.0×10-6～1.0×10-4 mol/L,检出限为8.0×10-7 mol/L.在测定实际样品的尿酸含量实验中,回收率为 99.7%～104.1%,结果满意.
Abstract
Citrulline is polymerized on a glassy carbon electrode through the cyclic voltammetry process to obtain a polycitrulline modified glassy carbon electrode(PCit/GCE).The electrochemical behaviors of uric acid on PCit/GCE are investigated.It is observed that the oxidation peak current of uric acid on PCit/GCE electrode exhibits a notable enhancement compared to that on the unmodified glassy carbon electrode,leading to a remarked improvement in sensitivity.The concentration of uric acid in the range of 2.0×10-6-1.0×10-4 mol·L-1 exhibits a robust linear correlation with its oxidation peak current.Additionally,the method achieves a low detection limit of 8.0×10-7 mol·L-1.In the determination of actual samples,the recovery rate falls within the range of 99.7%to 104.1%,showing a satisfactory result.
NETL
NSTL
万方数据