首页|虎杖根不同溶剂提取物的抗氧化活性研究

虎杖根不同溶剂提取物的抗氧化活性研究

Study on antioxidant activity of polygonum cuspidatum root extracts by different solvents

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为探究虎杖根6种不同溶剂提取物的抗氧化活性,利用1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除法、2,2-二氮-双(3-乙基苯并噻唑-6-磺酸)铵盐(ABTS)自由基清除法和总还原力检测法对6种不同溶剂虎杖根提取物进行抗氧化能力评价.选用抗氧化活性较强的95%乙醇提取物进行细胞层面内源性抗氧化能力的研究,评价其对抗氧化基因Nrf2、SOD、CAT、HO-1的调控作用.结果表明,相较于其他5种提取物,95%乙醇提取物的抗氧化能力最强且具有最高的多酚含量.95%乙醇提取物可显著增加Nrf2、SOD、CAT、HO-1等抗氧化基因的表达.95%虎杖根乙醇提取物具有较好的抗氧化活性,具有开发成为天然抗氧化剂的潜力.
The antioxidant ability of the extracts from polygonum cuspidatum root by six kinds of solvent respectively is evaluated by means of 1,1-diphenyl-2-picrylhydrazyl radical scavenging method,2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)radical scavenging method and total reduction power assay.The 95%-ethanol extract with high antioxidant activity is selected for the study of endogenous antioxidant capacity at the cellular level,and its effect on the regulation of antioxidant genes such as Nrf2,SOD,CAT and HO-1 is evaluated.It is found that the 95%-ethanol extract shows the highest antioxidant capacity and contains the highest polyphenol content compared to the other five extracts.The 95%-ethanol extract can significantly increase the expression of antioxidant genes such as Nrf2,SOD,CAT and HO-1.In a sum,the 95%-ethanol extract of polygonum cuspidatum root has good antioxidant activity and shows the potential to be developed as a natural antioxidant.

polygonum cuspidatum rootextractsdifferent solventsantioxidant activityendogenous antioxidant

秦小桐、曹昱莹、居瑞军、林炎钧、杜雨蒙、彭效明、管洁、严晓强、杨思敏

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北京石油化工学院,恩泽生物质精细化工北京市重点实验室,北京 102617

虎杖根 提取物 不同溶剂 抗氧化活性 内源性抗氧化

北京市教委科技一般项目北京市教委科技一般项目国家级大学生创新创业训练计划国家自然科学基金北京石油化工学院校内学科平台建设项目北京石油化工学院交叉科研探索项目

KM202210017011KM2018100170022023J00045817034532019XK006BIPTCSF-019

2024

现代化工
中国化工信息中心

现代化工

CSTPCD北大核心
影响因子:0.553
ISSN:0253-4320
年,卷(期):2024.44(4)
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