现代化工2024,Vol.44Issue(z2) :234-240,245.DOI:10.16606/j.cnki.issn0253-4320.2024.S2.042

异养硝化-好氧反硝化菌群YQS脱氮及聚集特性研究

Study on nitrogen removal and aggregation characteristics of heterotrophic nitrifying and aerobic denitrifying bacteria YQS

杨欣雨 刘玉香
现代化工2024,Vol.44Issue(z2) :234-240,245.DOI:10.16606/j.cnki.issn0253-4320.2024.S2.042

异养硝化-好氧反硝化菌群YQS脱氮及聚集特性研究

Study on nitrogen removal and aggregation characteristics of heterotrophic nitrifying and aerobic denitrifying bacteria YQS

杨欣雨 1刘玉香1
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作者信息

  • 1. 太原理工大学环境科学与工程学院,山西太原 030001
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摘要

从自然环境中分离得到一个兼具异养硝化-好氧反硝化(HN-AD)性能和聚集性能的菌群YQS,鉴定其优势属为Rhizobium、Azoarcu和Hydrogenophaga.综合分析了菌群YQS的脱氮性能、影响异养硝化性能的因素、聚集性能和胞外聚合物(EPS)的分泌情况.结果表明,菌群YQS对氨氮、硝态氮和亚硝态氮的去除率分别为97.33%、89.55%和85.94%;碳源为柠檬酸钠、C/N为12、温度为30℃、转速为130 r/min是菌群YQS进行异养硝化的最佳条件;菌群YQS的良好聚集性能与其分泌的EPS有关,聚集指数可达53.72%.菌群YQS的分离为实现污水生物高效脱氮和功能微生物富集提供了新的生物资源.

Abstract

A bacterial group YQS with both heterotrophic nitrification-aerobic denitrification(HN-AD)and aggregation properties was isolated from the natural environment,and its dominant genera were identified as Rhizobium,Azoarcu and Hydrogenophaga.The nitrogen removal performance,factors affecting heterotrophic nitrification performance,aggregation performance and extracellular polymer(EPS)secretion of YQS were analyzed comprehensively.The results showed that the removal rates of ammonia nitrogen,nitrate nitrogen and nitrite nitrogen by YQS were 97.33%、89.55%and 85.94%,respectively.The optimal conditions for heterotrophic nitrification were sodium citrate as carbon source,C/N of 12,temperature of 30℃ and rotation speed of 130 r/min.The good aggregation performance of YQS was related to the secretion of EPS,and the aggregation index reached 53.72%.The isolation of YQS provides a new biological resource for efficient nitrogen removal and functional microbial enrichment in wastewater.

关键词

异养硝化-好氧反硝化/根瘤菌/脱氮/聚集/胞外聚合物

Key words

HN-AD/Rhizobium/nitrogen removal/aggregation/EPS

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基金项目

山西省基础研究计划(202103021224106)

出版年

2024
现代化工
中国化工信息中心

现代化工

CSTPCD北大核心
影响因子:0.553
ISSN:0253-4320
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