首页|敲除H2-eb1基因建立的变应性鼻炎模型小鼠

敲除H2-eb1基因建立的变应性鼻炎模型小鼠

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
背景：HLA-DRB1与变应性鼻炎发病发病有关，构建HLA-DRB1基因敲除动物模型，不仅为阐明变应性鼻炎发病机制、同时也为相关疾病发病机制的阐明提供了良好的途径，然而未查阅到利用H2-eb1基因敲除小鼠进行相关研究的报道。目的：构建HLA-DRB1基因敲除动物模型。方法：经杂合子小鼠近亲繁殖，获得纯合子、野生型和杂合子小鼠。经基因及蛋白鉴定确认，采用随机数字表法选取8周龄雌性野生型(H2-eb1+/+)小鼠12只和 H2-eb1-/-小鼠12只，将12只 H2-eb1+/+小鼠和12只H2-eb1-/-小鼠以卵清蛋白致敏激发，建立小鼠变应性鼻炎模型。将另12只H2-eb1+/+小鼠以PBS代替卵清蛋白激发作为对照。结果与结论：与对照小鼠相比，变应性鼻炎模型小鼠血清中卵清蛋白IgE、白细胞介素4水平明显升高，γ-干扰素水平明显降低；而与基因敲除野生型小鼠(H2-eb1+/+)相比，基因敲除H2-eb1-/-变应性鼻炎小鼠IgE、白细胞介素4水平较低，γ-干扰素水平较高。提示H2-eb1基因在变应性鼻炎的发病机制中的Th1/Th2失衡有重要调节作用。
Establishing mouse models of allergic rhinitis by knocking outH2-eb1 gene
BACKGROUND:HLA-DRB1 is related to the pathogenesis of alergic rhinitis. Construction ofHLA-DRB1 gene knockout animal models not only elucidates the pathogenesis of alergic rhinitis, but also provides a good way for the elucidation of the pathogenesis of alergic rhinitis-related diseases. OBJECTIVE:To establish the HLA-DRB1gene knockout animal models. METHODS:Homozygous, wild-type and heterozygous mice were obtained by inbreeding of the heterozygous mice. Confirmed by gene and protein identification, 24 female wild-type (H2-eb1+/+) mice and 12 H2-eb1-/-mice aged 8 weeks were selected according to the random number table. 12 H2-eb1+/+ mice and 12 H2-eb1-/- mice were sensitized with ovalbumin to establish the mouse models of alergic rhinitis. Another 12 mice were sensitized with PBS as comparison. RESULTS AND CONCLUSION:Compared with the control mice, serum levels of ovalbumin-specific IgE and interleukin-4 were significantly increased, while serum level ofγ-interferon was significantly decreased in the mouse models of alergic rhinitis. Serum levels of IgE and interleukin-4 were lower, while serumγ- interferon level was higher, inH2-eb1-/-gene knockout mice of alergic rhinitis than those in the H2-eb1+/+ gene knockout wild-type mice. These results suggest thatH2-eb1 gene may play an important role in regulating Th1/Th2 imbalance in the pathogenesis of alergic rhinitis.

Rhinitis, Alergic, PerennialGene Knockout TechniquesInbreedingTh1/Th2 Balance Funding:the National Natural Science Foundation of China, No. 81160125

李林格、冯娟、胡斌、寿玺、张春、田钰、江春荣、张瑜、张华

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新疆医科大学第一附属医院耳鼻喉科，新疆维吾尔自治区乌鲁木齐市 830054

实验动物 基因病毒载体及相关因子动物模型 变态反应性疾病 变应性鼻炎 基因敲除 基因型聚合酶链反应 免疫印迹 近亲繁殖 Th1/Th2平衡 国家自然科学基金

2015

10.3969/j.issn.2095-4344.2015.27.028

中国组织工程研究
中国康复医学会,《中国组织工程研究与临床康复》杂志社

中国组织工程研究

CSTPCD北大核心
影响因子：1.387
ISSN：2095-4344
年,卷(期)：2015.(27)
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