Objective To evaluate the adhesion characteristic of Lactobacillus acidophilus (La) with different states to KB cell line and their competition inhibition effects on Porphyromonas gingivalis (Pg) and Fusobacterium nuclearum (Fn). Methods For the La adherence test, KB cell cultured on cover-slips incubated with La4356 were tested for 1h, 3h, 6h, 12h, 24h and 48h at 37℃. After the incubation, the cover-slips were fixed with raethanol and stained with Grams stain. Fifty cells were counted for each specimen under the microscope. For the antagonistic test, the suspension of Pg, Fn mixed with La of different states were cultured with KB cell had to be cultivated together for lh, 50 invaded cells were counted for each specimen under the microscope, too. The data were analyzed by t test using SPSS 13.0 software package. Results Both live and heat-killed La could adhere to KB cell and inhibit the growth of Pg and Fn (P<0.05), and there was slight difference between live and heat-killed. The adhesion of live La is much higher than the heat-killed. Both live and heat-killed La can significantly reduce the adhesion of Pg and Fn, and the group with La of high concentration shows a more stronger antagonism effect. The heat -killed La demonstrates a more significant antagonistic effect when its concentration is more than the pathogens (over 1:1). Conclusion Both live and heat-killed La can adhere to KB cells and inhibit the adhesion of Pg and Fn. The new strategy of confining periodontal pathogens with La as ecological modulator is worthy of further study.
NETL
NSTL
万方数据
维普
