A preliminary study of quercetin in the prevention of periodontal lesions in smokers through JAK/STAT signal pathway
Objective To explore the effect of quercetin on the ability of cell migration,secretion of collagen Ⅰ(COL Ⅰ)and collagen Ⅲ(COL Ⅲ),mRNA expression of interleukin(IL)-1β,tumor necrosis factor(TNF)-a,cyclooxygenase(COX)-2 of human periodontal ligament fibroblasts(HPLFs)in an inflammatory environment by establishing inflammation and inflammation with smoking model in vitro.And to explore the effect of quercetin on JAK/STAT signal pathway.Methods HPLFs was cultured and identified,and cigarette smoke extract(CSE)and lipopolysaccharide(LPS)were used to establish inflammation and inflammation with smoking model.The cells were divided into 8 groups:normal group(N),CSE group(S),lipopolysaccharide group(L),lipopolysaccharide+JAK/STAT blocker group(L+AG490),lipopolysaccharide+CSE group(L+S),lipopolysaccharide+CSE+JAK/STAT blocker group(L+S+AG490),lipopolysaccharide+quercetin group(L+Q),lipopolysaccharide+CSE+quercetin group(L+S+Q).The ability of cell migration,collagen secretion,expression of inflammatory cytokines and p-STAT3 were detected by scratch test,ELISA,RT-PCR and immunofluorescence staining respectively.Results Compared with group N,the ability of cell migration and collagen secretion decreased,the expression of inflammatory cytokines and p-STAT3 increased in group L,S and L+S(P＜0.05).The ability of cell migration and collagen secretion increased in group L+AG490 and L+Q compared with group L,while the expression of inflammatory factors and p-STAT3 decreased(P＜0.05).The ability of cell migration and collagen secretion increased in group L+S+AG490 and L+S+Q compared with group L+S,while the expression of inflammatory factors and p-STAT3 decreased(P＜0.05).Conclusion Cigarette smoke extract and lipopolysaccharide can inhibit the migration ability of HPLFs,induce the expression of inflammatory factors,inhibit collagen secretion and promote the expression of p-STAT3,while AG490 and quercetin protect HPLFs and inhibit the expression of p-STAT3.It is speculated that quercetin may protect HPLFs by inhibiting JAK/STAT pathway.
Human periodontal ligament fibroblastsJAK/STAT signal pathwayCigarette smoke extractQuercetinPeriodontitis
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