How to eliminate the interference of high IgM in serum on uric acid and prealbumin detection
Objective:To try to find out a more convenient method to reduce the interference of higher concentration of IgM on uric acid(UA)and prealbumin(PA)in routine wet biochemical tests.Method:Polyethylene glycol(25%PEG),deionized water(DH2O),and saline(0.9%NaCl)were used to dilute the specimens,which were centrifuged for 10 minutes using 3000 rpm and 10,000 rpm,respectively,and the supernatants were taken for testing,and the results were multiplied by the number of dilutions.Result:Dilution centrifugation using 25%PEG or DH2O effectively eliminated the interference of high IgM on UA and PA,while the use of saline was ineffective.Conclusion:The use of 25%PEG*10,000 rpm,DH2O*3000 and 10,000 rpm in high IgM specimens eliminated their detection of UA and PA.
Polyethylene glycoluric acidprealbuminimmunoglobulin M
万方数据
维普
