Determination of 4 proprietary Chinese medicines in Lipophilic Shengfa tablets by HPLC wavelength switching method
Objective:A HPLC wavelength switching method was established for the determination of four components in Chuzhi Shengfa Tablets,including Humidone.Method:Agilent Eclipse Plus C18(4.6×250 mm,5 µm)was used.The mobile phase was consisted of acetonitrile(A)-0.3% phosphoric acid solution(B)with gradient elution.The elution procedures were:0～6 min 89% A,6～27 min 89%～31% A,27～30 min 31%～89% A,31～35 min 89% A.Column temperature:30°C,column flow:1.0 mL/min.Detection wavelengths of paeonol,xibutanone,5-O-methylvisamitol side and humenthone were set at 274 nm、236 nm、254 nm and 252 nm,respectively.Injection volume was 10 µL.HPLC-DAD for quantitative detection Result:Paeonol,obacunone,5-O-methylvisamitol side and pulegone showed good linear relationships in concentration range of 0.01996～1.996、0.5868～5.868、0.997～9.97、0.0998～9.98 μg/mL.The average recoveries were 100.92%、100.54%、101.14%、100.93%,respectively.Precision and reproducibility of the method were within 5.0% .Conclusion:This method has the advantages of simple pretreatment,and is suitable for the quality control of lipophaga Shengfa tablets.
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