基于HMGB1通路的盐酸戊乙奎醚对脑梗死的保护效应
The Protective Effect of Penehyclidine Hydrochloride on Cerebral Infarction via the HMGB1 Pathway
梁振生 1张梦翔 1杨晓丹 1盛竹君 1张楠 1杨满琴 1王保国 2谢若男1
作者信息
- 1. 安徽中医药大学第二附属医院药剂科,安徽合肥 230061
- 2. 安徽中医药大学第二附属医院脑病六科,安徽合肥 230061
- 折叠
摘要
目的:探究基于HMGB1通路的盐酸戊乙奎醚对脑梗死的保护效应.方法:取 50只SD雄性大鼠分组探讨PHC治疗脑梗死的最佳剂量及效果,并探究HMGB1 通路的作用.结果:PHC干预可减轻神经细胞损伤,效果与剂量相关.与假手术组相比,模型组和 PHC组多项指标异常;而与模型组相比,PHC组指标改善,且与剂量相关.抑制HMGB1与PHC高剂量效果相同,可改善脑组织炎症和氧化应激,降低蛋白表达.PHC治疗与抑制HMGB1/TLR4/NF-κB通路相关,联合抑制可增强效果.结论:PHC对脑梗死大鼠具有保护作用,抑制HMGB1/TLR4/NF-κB通路,下调炎症因子表达.
Abstract
Objective:To investigate the protective effect of penehyclidine hydrochloride on cerebral infarction based on the HMGB1 pathway.Methods:50 SD male rats were grouped to investigate the optimal dosage and effect of PHC in treating cerebral infarction,as well as to explore the role of the HMGB1 pathway.Results:PHC intervention can alleviate nerve cell damage,and its effect is correlated with the dosage.Compared with the sham operation group,multiple indicators in the model group and the PHC group are abnormal.However,compared with the model group,the indicators in the PHC group are improved,and the improvement is also related to the dosage.Inhibition of HMGB1 has the same effect as a high dose of PHC,which can improve brain tissue inflammation and oxidative stress,and reduce protein expression.PHC treatment is related to the inhibition of the HMGB1/TLR4/NF-κB pathway,and combined inhibition can enhance the effect..Conclusions:PHC has a protective effect on rats with cerebral infarction by inhibiting the HMGB1/TLR4/NF-κB pathway and downregulating the expression of inflammatory factors.
关键词
盐酸戊乙奎醚/高迁移率组蛋白B1/Toll样受体4/核因子-κB/脑梗死Key words
Penehyclidine hydrochloride/High mobility group histone B1/Toll-like receptor 4/Nuclear factor-κB/Cerebral infarction引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据