经长春海关关区输入的境外新型冠状病毒基因组特征分析
Analysis of genomic characteristics of SARS-CoV-2 imported from abroad through Changchun Customs
丁旭 1张井巍 2王玮琳 1马文晨 1刘韬 1石洋 1孙丽华 1张婷1
作者信息
- 1. 吉林国际旅行卫生保健中心,长春 130062
- 2. 临沧市疾病预防控制中心,临沧 677000
- 折叠
摘要
为了解经长春海关关区输入的新型冠状病毒肺炎(Coronavirus Disease 2019,COVID-19)感染者人口学特征和新型冠状病毒(SARS-CoV-2)全基因组变异情况.收集 2023 年 4 月至 12 月经长春海关关区输入的 27 例新型冠状肺炎感染者个案信息.采用RT-PCR方法对咽拭子样本进行核酸检测,CT值中ORF1ab基因和N基因Ct值均在25 至 30 之间,占 55.56%.应用第三代纳米孔基因测序仪测序及新型冠状病毒全基因组分析软件V1.0 分析,测序覆盖度为 98.02%～99.59%.与武汉参考株(NC_045512.2)相比SARS-CoV-2基因组序列核苷酸突变位点数在 62～132个之间.Pangolin分型显示均为Omicron变异株,EG.5.1及其亚分支占比最多,占 25.93%.通过分析经长春海关关区入境的人员新冠病毒核酸及基因序列,监测境外SARS-CoV-2输入情况,可实时掌握病毒株变异趋势.
Abstract
To understand the demographic characteristics of imported patients with Coronavirus Disease 2019(COVID-19)and the whole genome variation of SARS-CoV-2 through Changchun Customs.The case information of 27 patients with COVID-19 imported through Changchun Customsfrom April to December 2023 was collected.The RT-PCR method was used to detect the nucleic acid of respiratory tract samples.The Ct values of ORF1ab gene and N gene were between 25 and 30,accounting for 55.56%.The whole genome of the virus was analyzed by the third-generation nanopore gene sequencer sequencing and the whole genome analysis V1.0 software,and the sequencing coverage was 98.02%-99.59%.Compared with the Wuhan reference strain(NC_045512.2),the number of nucleotide mutation sites in the SARS-CoV-2 genome sequence was between 62 and 132.Pangolin typing showed that all of them were Omicron mutant strains,EG.5.1 and its sub-branches accounted for the largest proportion,accounting for 25.93%.By analyzing the nucleic acid and gene sequence of the novel coronavirus of entry personnel at the ports through Changchun Customs and monitoring the import of SARS-CoV-2 abroad,the variation trend of the virus strain can be grasped in real time.
关键词
新型冠状病毒/Omicron变异株/境外输入/全基因组三代测序Key words
SARS-CoV-2/Omicron variant strain/Imported cases/Whole genome third-generation sequencing引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据