High-sensitivity quantitative determination of Escherichia coli O157:H7/NM in vegetable by Droplet Digital PCR
A rapid and accurate method for the detection of Escherichia coli O157:H7/NM by microdrop digital polymerase chain reaction(droplet digital PCR)in vegetables was established to evaluate its feasibility.A microdrop digital PCR system was established by using specific primers and probes of rfbE gene.The detection limit of microdrop digital PCR for Escherichia coli O157:H7/NM was as low as 1×103 CFU/mL pure bacterial solution,and this method had high specificity and sensitivity,stable repeatability results,and did not cross-react with other common pathogens.A quantitative method for detection of Escherichia coli O157:H7/NM in vegetables by microdrop digital PCR was established.
Droplet digital PCREscherichia coli O157H7/NMquantitative detection
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