Determination of 7 Organic Acids in fixed beverage by HPLC
A HPLC method was developed for the simultaneous determination of tartaric acid,lactic acid,malic acid,citric acid,succinic acid,fumaric acid and adipic acid in solid beverage.The samples were separated using a Purospher® STAR RP-18(5µm)Hibar®RT 250-4.6 column with mobile phases of 0.1%phosphoric acid and methanol at a flow rate of 1.0 mL/min and a temperature of 30℃,and quantified by external standard method.The correlation coefficient R2 of calibration curves of 7 organic acids was above 0.9990.The RSD range of the method was 2.8%to 8.6%(n=6),and the recoveries were 91.9%to 108%.The results show that the method is simple,accurate and reproducible,and can be used for the determination of organic acids in food.
High performance liquid chromatographyOrganic acid solid beverage
万方数据
维普
