Experimental Study on Restoration of Acid Mine Drainage by Fungi
In order to study the remediation potential of biological methods on acid mine wastewater,aiming at the shortcomings of traditional neutralization method in the treatment of acid mine wastewater,such as high treatment cost,easy to cause secondary pollution,and not conducive to the sustainable devel-opment of social economy and ecological environment,the experimental study of fungal remediation of acid mine wastewater is carried out.Firstly,fungi were separated from acid mine wastewater,and then the fungi were identified by morphology and 18 s rDNA.The effects of fungi on dissolved oxygen,ORP,phenol-phthalein acidity and pH value of acid mine wastewater were studied,and their removal effects on various metal ions and sulfate were analyzed.The results showed that the acid-resistant bacteria separated was As-pergillus sp.MF1.The biofilm formed by Aspergillus sp.MF1 could reduce the dissolved oxygen,redox po-tential and phenolphthalein acidity in wastewater,and increase the pH value of wastewater.The removal ef-ficiency of TFe,Zn2+,Cu2+,Cd2+is up to 79.39%,83.76%,97.10%and 99.01%,respectively.It shows that Aspergillus sp.MF1 and other microorganisms can increase the pH value of acid mine wastewater and reduce the concentration of metal ions,which has potential application value for repairing and treating acid mine wastewater.This study can provide material basis and practical basis for bioremediation of acid mine drainage.
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