Cu 2Se nanoparticles induce ferroptosis on gastric cancer cells through chemo-dynamic therapy
梁敏 1史洁云 2张翔
扫码查看
点击上方二维码区域，可以放大扫码查看
作者信息
1. 1广州医科大学附属第五医院肿瘤科，广州 510700
2. 2广州医科大学附属第五医院社区中心，广州 510700
折叠
摘要
目的 探讨硒化亚铜（Cu2Se）纳米的制备、物化表征及其对胃癌的抗肿瘤效应。 方法 采用透射电镜，水合粒径分析，Zeta电位分析及紫外吸收光谱分析方法分析Cu2Se纳米的形貌、粒径大小及类芬顿性能，同时采用激光共聚焦、细胞毒性（CCK-8）、Transwell实验评估该纳米的体外抗肿瘤作用效应毒性，最后构建单侧皮下荷瘤模型探究Cu2Se纳米的体内抗肿瘤作用。 结果 制备的Cu2Se纳米为100 nm左右的类圆形中空结构，可催化过氧化氢生成单线氧。激光共聚焦实验表明Cu2Se可以促进胃癌细胞活性氧生成，诱导铁死亡发生；CCK-8实验表明，Cu2Se纳米对胃癌细胞具有浓度依赖性抑制增殖作用；Transwell实验表明，Cu2Se纳米能抑制胃癌细胞的侵袭迁移能力；裸鼠单侧皮下荷瘤模型表明Cu2Se纳米能安全高效抑制肿瘤增殖。 结论 Cu2Se纳米具有良好的类芬顿效能，可诱导胃癌铁死亡发生，具备良好的体内外抑制抗肿瘤效应，是胃癌治疗的一种潜在新方法。 Objective To investigate the preparation, physicochemical characterization of cuprous selenide nanoparticles (Cu2Se NPs), and their antitumor effect on gastric cancer (GC) . Methods The morphology, size and Fenton-like properties of Cu2Se NPs were analyzed by transmission electron microscopy (TEM), size distributions, Zeta potential and UV absorption spectroscopy. Meanwhile, confocal laser scanning microscopy (CLSM), Cell Counting Kit-8 (CCK-8) and Transwell experiments were used to evaluate the in vitro antitumor effect of Cu2Se NPs. Finally, subcutaneous tumor-bearing mice were constructed to explore the in vivo antitumor effect of Cu2Se NPs. Results Our as-designed Cu2Se NPs showed quasi-circular hollow structures with ~100 nm, which could catalyze the generation of singlet oxygen from hydrogen peroxide. CLSM results showed that Cu2Se could help generate reactive oxygen species (ROS) and induce ferroptosis in GC cells. CCK-8 results showed that Cu2Se had a concentration-dependent inhibitory effect on the proliferation of GC ells. Transwell experiments showed that Cu2Se could suppress the migration and invasion capacities of GC cells. Tumor-bearing nude mice demonstrated that Cu2Se could efficiently reduce proliferation of GC with high safety. Conclusion Cu2Se NPs have good Fenton-like efficacy, which could induce ferroptosis, and showed good inhibitory effects on GC in vitro and in vivo. Therefore, our research serves a potential novel method for GC treatment.
Abstract
Objective To investigate the preparation, physicochemical characterization of cuprous selenide nanoparticles (Cu2Se NPs), and their antitumor effect on gastric cancer (GC) . Methods The morphology, size and Fenton-like properties of Cu2Se NPs were analyzed by transmission electron microscopy (TEM), size distributions, Zeta potential and UV absorption spectroscopy. Meanwhile, confocal laser scanning microscopy (CLSM), Cell Counting Kit-8 (CCK-8) and Transwell experiments were used to evaluate the in vitro antitumor effect of Cu2Se NPs. Finally, subcutaneous tumor-bearing mice were constructed to explore the in vivo antitumor effect of Cu2Se NPs. Results Our as-designed Cu2Se NPs showed quasi-circular hollow structures with ~100 nm, which could catalyze the generation of singlet oxygen from hydrogen peroxide. CLSM results showed that Cu2Se could help generate reactive oxygen species (ROS) and induce ferroptosis in GC cells. CCK-8 results showed that Cu2Se had a concentration-dependent inhibitory effect on the proliferation of GC ells. Transwell experiments showed that Cu2Se could suppress the migration and invasion capacities of GC cells. Tumor-bearing nude mice demonstrated that Cu2Se could efficiently reduce proliferation of GC with high safety. Conclusion Cu2Se NPs have good Fenton-like efficacy, which could induce ferroptosis, and showed good inhibitory effects on GC in vitro and in vivo. Therefore, our research serves a potential novel method for GC treatment.
关键词
硒化亚铜/纳米药物/化学动力疗法/铁死亡
Key words
Cu 2Se/Nanomedicine/Chemodynamic Therapy/Ferroptosis
万方数据