摘要
目的 探讨辅助性T细胞9(Th9)、Th17及Th22相关细胞因子白细胞介素-9(IL-9)、IL-17及IL-22与2型糖尿病(T2DM)患者并发骨质疏松症(OP)的关系.方法 选取2020年4月-2023年2月在长治医学院附属和济医院、和平医院内分泌科及长治市潞州区东街街道社区卫生服务中心就诊的104例T2DM并发OP患者作为并发组,另选取同期60例单纯T2DM患者作为单纯T2DM组.采用双能X线检测两组L1～4椎体骨密度(BMD)值;采用流式细胞仪检测两组外周血中Th9、Th17及Th22细胞水平;采用酶联免疫吸附法(ELISA)检测两组血清IL-9、IL-17、IL-22水平.经Pearson相关系数分析T2DM并发OP患者外周血中IL-9、IL-17、IL-22水平与骨密度的相关性;以多因素Logistic回归分析T2DM患者并发OP的独立危险因素.结果 Pearson相关性分析显示,T2DM并发OP患者血清中IL-9、IL-17、IL-22与骨密度均呈负相关(r=-0.337,-0.459,-0.310,P＜0.001);多因素Logistic回归分析结果显示,TRAP-5b、BMI、L1～4BMD、Th9、Th17、Th22 细胞及血清 IL-9、IL-17、IL-22 是 T2DM 患者并发 OP 的独立影响因素.结论 Th9、Th17及Th22细胞可能通过分泌IL-9、IL-17、IL-22促进T2DM患者骨质疏松症的发生,干预IL-9、IL-17、IL-22的高表达有望成为治疗T2DM患者骨质疏松的潜在靶点.
Abstract
Objective To investigate the relationship between T helper cell 9(Th9),Th17 and Th22 related cytokines interleukin-9(IL-9),IL-17 and IL-22 and osteoporosis(OP)in patients with type 2 diabetes mellitus(T2DM).Methods From April 2020 to February 2023,104 patients with T2DM complicated with OP who were treated in the Department of Endocrinology,Heji Hospital,Changzhi Medical College and Dongjie Street Community Health Service Center,Changzhi City were selected as the concurrent group,and 60 patients with simple T2DM were selected as the simple T2DM group.Bone mineral density(BMD)of L1-4 vertebral body was detected by dual energy X-ray.The levels of Th9,Th17 and Th22 cells in peripheral blood of the two groups were detected by flow cytometry.The levels of serum IL-9,IL-17 and IL-22 were detected by enzyme-linked immunosorbent assay(ELISA).Pearson correlation coefficient was used to analyze the correlation between IL-9,IL-17,IL-22 levels in peripheral blood and bone mineral density in T2 DM patients with OP.Multivariate Logistic regression analysis was used to analyze the independent risk factors of OP in T2 DM patients.Results Pearson correlation analysis showed that serum IL-9,IL-17 and IL-22 in T2 DM patients with OP were negatively correlated with BMD(r=-0.337,-0.459,-0.310,P＜0.001).Multivariate Logistic regression analysis showed that TRAP-5b,BMI,L1-4BMD,Th9,Th17,Th22 cells and serum IL-9,IL-17,IL-22 were independent influencing factors of OP in T2DM patients.Conclusion Th9,Th17 and Th22 cells may promote the occurrence of osteoporosis in T2 DM patients by secreting IL-9,IL-17 and IL-22.Intervention of the high expression of IL-9,IL-17 and IL-22 is expected to become a potential target for the treatment of osteoporosis in T2 DM patients.
基金项目
山西省科技厅基础研究计划项目基金(202103021223012)
NETL
NSTL
万方数据