KDM6B通过MAPK信号通路调控肾癌细胞的增殖和迁移
Effects of the biological function of KDM6B on clear cell renal cell carcinoma
林昌伟 1袁祖君1
作者信息
- 1. 637000 南充,川北医学院附属南充市中心医院肾病内科
- 折叠
摘要
目的 研究赖氨酸特异性去甲基酶 6 B(KDM6 B)表达对肾透明细胞癌增殖和迁移侵袭的影响.方法 通过基因表达谱交互分析数据库分析 KDM6 B表达与肾透明细胞癌临床分期与预后的关系.将肾透明细胞癌患者的肿瘤组织石蜡切片进行 KDM6 B免疫组化染色并统计与临床病理特征的相关性,使用敲低对照小干扰 RNA 以及敲低 KDM6 B小干扰 RNA 对人肾透明细胞癌细胞株 ACHN 和 Caki-1 细胞分别进行转染,使用实时荧光定量聚合酶链式反应(RT-qPCR)及Western blot验证转染效率,使用CCK8 实验检测转染后肾透明细胞癌细胞增殖能力的变化,克隆形成实验检测细胞形成克隆的能力,使用 Transwell 实验检测细胞迁移侵袭能力的变化.使用Western blot实验检测丝裂原活化蛋白激酶(MAPK)信号通路中磷酸化的细胞外信号调节激酶蛋白(p-ERK)及细胞外信号调节激酶蛋白的表达水平.结果 肾透明细胞癌组织中 KDM6 B 的高表达与良好的疾病分期与预后密切相关.在两种细胞系中转染 siKDM6B 均能成功敲低 KDM6 B的表达.敲低KDM6 B表达后肾透明细胞癌细胞 ACHN和Caki-1 的细胞增殖能力显著增强,细胞克隆形成能力明显增强,细胞迁移侵袭能力显著增强.敲低 KDM6 B表达后 p-ERK 表达水平显著增加.结论 KDM6 B抑制 MAPK信号通路激活及肾透明细胞癌细胞进展,该蛋白可能成为诊断肾透明细胞癌的生物标记物以及治疗肾透明细胞癌的潜在靶点.
Abstract
Objective To study the effect of lysine specific demethylase 6 B(KDM6 B)expres-sion on the proliferation,migration and invasion of clear cell renal cell carcinoma(ccRCC).Meth-ods GEPIA website was used to analyze the relationship between KDM6 B expression and pathologi-cal stage and prognosis of ccRCC.Human ccRCC cell lines ACHN and Caki-1 were cultured,and siNC and siKDM6 B small interfering RNA were synthesized.ACHN and Caki-1 cells were transfect-ed by siRNA.The transfection efficiency of ACHN and Caki-1 cells was verified by RT-qPCR and Western blo.t The proliferation ability of the transfected ccRCC cells was detected by CCK8 as-say.Colony formation assay was used to detect the ability of cells to form clones,and transwell assay was used to detect the migration and invasion of cancer cells.Western blot assay was used to detect MAPK signaling pathway and its changes.Results The high expression of KDM6 B in patients with ccRCC was closely related to earlier disease stage and better prognosis.The expression of KDM6 B was successfully knocked down by siKDM6 B in both cell lines.Knockdown of KDM6 B ex-pression significantly enhanced the proliferation ability,cell clonogenesis ability,and cell migration and invasion ability of ccRCC cells ACHN and Caki-1.After knockdown of KDM6 B expression,p-ERK expression level increased significantly.Conclusions KDM6 B suppresses the MAPK signa-ling pathway and the cancer progression in ccRCC,which may be a biomarker for the diagnosis of ccRCC and a potential target for the treatment of ccRCC.
关键词
赖氨酸特异性去甲基酶6B/肾透明细胞癌/细胞增殖/MAPK信号通路Key words
KDM6 B/Clear cell renal carcinoma/Cell proliferation/MAPK signaling pathway引用本文复制引用
出版年
2024
NETL
NSTL
万方数据