摘要
目的 检测膀胱癌组织长链非编码核糖核酸(LncRNA)丝氨酸肽酶抑制剂Kunitz 1型反义核糖核酸1(SPINT1-AS1)表达并探讨其临床意义.方法 选取安阳市第三人民医院泌尿外科2018年1月至2023年2月收治的328例行腹腔镜下根治术膀胱癌患者作为研究对象,RT-qPCR检测膀胱癌组织和切缘正常组织LncRNA SPINT1-AS1的表达.比较不同临床病理特征患者癌组织LncRNA SPINT1-AS1的表达;随访至2023年5月,分析膀胱癌组织LncRNA SPINT1-AS1的表达与腹腔镜下根治术后复发的关系;Cox回归分析探讨影响膀胱癌患者腹腔镜下根治术后复发的危险因素.结果 LncRNA SPINT1-AS1在膀胱癌组织的表达高于切缘正常组织(P＜0.05);肌层浸润、TNM分期≥Ⅱb期、最大肿瘤直径≥3 cm、多发病灶、有淋巴结转移患者膀胱癌组织LncRNA SPINT1-AS1的表达分别高于非肌层浸润性、TNM分期＜Ⅱ b期、最大肿瘤直径＜3 cm、单发病灶、无淋巴结转移患者(P＜0.05);随访期间患者腹腔镜下根治术后复发率为15.88％(47/296);肌层浸润(HR=1.692,95％CI:1.157～2.475)、TNM 分期≥ Ⅱ b 期(HR=1.784,95％CI:1.187～2.682)、多发病灶(HR=1.837,95％CI:1.200～2.810)、膀胱癌组织 LncRNA SPINT1-AS1表达(HR=1.557,95％CI:1.195～2.029)均为腹腔镜下根治术后复发的危险因素(P＜0.05).结论 膀胱癌组织LncRNA SPINT1-AS1表达高于切缘正常组织,肌层浸润、TNM分期≥ Ⅱ b期、多发病灶及癌组织LncRNA SPINT1-AS1表达均为膀胱癌腹腔镜下根治术后复发的危险因素.
Abstract
Objective To detect the expression of Kunitz type 1 Antisense RNA 1(SPINT1-AS1),a serine peptidase inhibitor of long non-coding RNA(LncRNA)in bladder cancer tissues and explore its clinical significance.Methods A total of 328 patients with bladder cancer undergoing laparoscopic radical surgery admitted to our unit from January 2018 to February 2023 were selected as research objects.The expressions of LncRNA SPINT1-AS1 in bladder cancer tissues and normal incisal margin tissues were detected by reverse transcription real-time fluorescence quantitative poly-merase chain reaction(RT-qPCR).The expressions of LncRNA SPINT1-AS1 in cancer tissues of patients with different clinicopathological features were compared.Following-up was conducted until May 2023.The relationship between LncRNA SPINT1-AS1 expression and recurrence after laparo-scopic radical surgery in bladder cancer.Cox regression analysis was conducted to investigate the risk factors in recurrence for patients with bladder cancer after radical laparoscopic surgery.Results The expression of LncRNA SPINT1-AS1 in bladder cancer tissues was higher than that in normal in-cisal margin tissues(P＜0.05).The expressions of LncRNA SPINT1-AS1 in bladder cancer in pa-tients with muscle invasion,TNM stage Ⅱb,maximum tumor diameter ≥3 cm,multiple le-sions,and lymph node metastases were all higher than those in patients with non-muscle invasion,TNM stage＜Ⅱ b,maximum tumor diameter＜3 cm,single lesion,and no lymph node metastases,respectively(P＜0.05).During the follow-up period,the recurrence rate of patients after radical laparoscopic surgery was 15.88％(47/296).Muscle infiltration(HR=1.692,95％CI:1.157-2.475),TNM stage ≥ Ⅱ b stage(HR=1.784,95％CI:1.187-2.682),multiple lesions(HR=1.837,95％CI:1.200-2.810)and LncRNA SPINT1-AS1 expression in bladder cancer tissues(HR=1.557,95％CI:1.195-2.029)were risk factors in recurrence after laparoscopic radical surgery(P＜0.05).Conclusions The ex-pression of LncRNA SPINT1-AS1 in bladder cancer tissue was higher than that in normal incisional margin tissue.Muscle in-vasion,TNM stage ≥ Ⅱ b,multiple lesions and LncRNA SPINT1-AS1 expression in cancer tissues were all risk factors in re-currence of bladder cancer after laparoscopic radical surgery.
维普
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