Objective To investigate the expression of mitogen-activated protein kinase kinase 1(MAP2K1)in clear cell renal cell carcinoma(ccRCC)and its effect on the proliferation,invasion and migration of ACHN cells.Methods From January 2011 to December 2019,ccRCC tissues surgically removed from 82 cases and matched para-cancer tissue specimens at Sinopharm Tongmei General Hospital were collected.Immunohistochemical staining was used to detect the expression level of MAP2K1 in 82 ccRCC cancer tissues and paired adjacent tissues,and to analyze the relation-ship between MAP2K1 expression and the clinicopathologic features and survival prognosis of ccRCC.The effects of silencing MAP2K1 gene on the biological function of ACHN cells were detec-ted by CCK-8 test,Transwell invasion and migration test.Results The expression rate of MAP2K1 in cancer tissues was higher than that in paracancer tissues(74.39％vs 23.17％,P＜0.001).The relative expression of MAP2K1 protein in cancer tissues was significantly higher than that in adjacent tissues[(0.89±0.10)vs(0.21±0.05),P=0.005].The expression level of MAP2K1 was correlated with TNM stage and T stage(P=0.04,P=0.030).The analysis of sur-vival curve showed that the median survival time of patients with high expression of MAP2K1 was 39.3 months,while that of patients with low expression of MAP2K1 was 87.6 months.The differ-ence between the two was statistically significant(P＜0.001).After MAP2K1 gene was silenced,MAP2K1 protein expression level(P=0.037),cell proliferation activity at 72 h(P=0.002),cell proliferation activity at 96 h(P=0.001),invasion force(P=0.014)and migration force(P=0.002)of ACHN cells de-creased.Conclusions The high expression of MAP2K1 in ccRCC cancer tissue indicates poor survival prognosis of patients.Silencing MAP2K1 gene can inhibit the proliferation,invasion and migration of ccRCC cancer cells.MAP2K1 may become a biological marker of ccRCC.
关键词
肾透明细胞癌/丝裂原活化蛋白激酶激酶1/预后/增殖/侵袭
Key words
Clear cell renall cell carcinoma/Mitogen activated protein kinase kinase 1/Prognosis/Proliferation/Invasion
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