Objective To investigate the regulatory mechanism of fatty acid desaturase 1(FADS1)for proliferation,migration and autophagy induced ferroptosis in bladder cancer cell lines T24 and 5637.Methods The expression of FADS1 in bladder cancer and its influence on patient prognosis were analyzed in The Cancer Genome Atlas.The bladder cancer cell line T24 and 5637 cells were cultured in vitro,then divided into control group and shRNA group after knockdown of FADS1 with shRNA.The effects of FADS1 knockdown on cell proliferation and migration were in-vestigated by plate cloning,migration and invasion experiments.The expressions of autophagy relat-ed proteins LC3-Ⅱ.P62 and BECN1,and ferroptosis related proteins NCOA4 and GXP4 in cells of each group were detected by Western blot.Results The expression of FADS1 in bladder cancer tissues was significantly higher than that in paracancer tissues,and its expression was correlated with lymph node metastasis and stage of tumor.Compared with patients with low expression of FADS1,the survival rate of patients with high expression of FADS1 was significantly lower.After knock-down of FADS1,cell proliferation decreased,autophagy related protein LC3-Ⅱ protein and BECN1 protein expression increased,P62 protein expression decreased,ferroptosis related protein NCOA4 protein expression increased,and GXP4 expression decreased.Conclusions After knockdown of FADS1,cell proliferation of bladder cancer cells decreased,autophagy was activated and ferroptosis was induced.FADS1 may play an important role in the occurrence and development of bladder canc-er,which provides a new theoretical basis for molecular targeted therapy of bladder cancer.
维普
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