A preliminary study on the resistance level and resistance mechanism to cyhalofop-butyl in Echinochloa crus-galli populations in rice field
In order to clarify the resistance level and resistance mechanism to cyhalofop-butyl in Echinochloa crus-galli populations from Hunan and Jiangxi paddy fields,the experimental studies were carried out by whole-plant bioassay,gene sequencing,and metabolic enzyme inhibitor coupled with cyhalofop-butyl.The results showed that R1,R2 and R3 populations displayed medium level resistance to cyhalofop-butyl,with the resistance indexs of 3.08,2.19 and 3.83,respectively.R4 population displayed low level resistance to cyhalofop-butyl,with the resistance index of 1.45.The Ile-2041-Val mutation was present in R1,and the Ile-2041-Val and Asp-2078-Gly mutations were present in R2,and malathion,NBD-C1 increased the sensibilities of R1 and R2 to cyhalofop-butyl.The Trp-2027-Arg mutation was present in R3 and NBD-C1 could increase the sensitivity to cyhalofop-butyl.The Cys-2088-Tyr mutation was present in R4,malathion and NBD-C1 couldn't reverse the resistance to cyhalofop-butyl.This study indicated that the medium level resistance of R1,R2,and R3 to cyhalofop-butyl was caused by a combination of target-site and non-target-site resistance,the low level resistance of R4 to cyhalofop-butyl was mainly mediated by target-site resistance.
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