现代农药2024,Vol.23Issue(6) :58-62,72.DOI:10.3969/j.issn.1671-5284.2024.06.009

农药降解菌的筛选及应用评价

Screening and application evaluation of pesticide degrading strain

孔洁 樊梅娜 陶宁 韩广泉 冉新炎 王丽荣
现代农药2024,Vol.23Issue(6) :58-62,72.DOI:10.3969/j.issn.1671-5284.2024.06.009

农药降解菌的筛选及应用评价

Screening and application evaluation of pesticide degrading strain

孔洁 1樊梅娜 1陶宁 1韩广泉 1冉新炎 1王丽荣1
扫码查看

作者信息

  • 1. 山东碧蓝生物科技有限公司/山东省农业微生物技术创新中心/黄淮海农业微生物资源与利用重点实验室,山东泰安 271000
  • 折叠

摘要

采用富集驯化、分离纯化法从受农药污染的土样和水样中筛选分离得到1株农药降解菌NBL-B0078,并通过形态学和生理生化特征,以及16S rRNA基因序列分析进行鉴定,同时,考察了其对作物的安全性,及对土壤和萝卜中农药残留的降解能力.结果表明,菌株NBL-B0078对百菌清、敌敌畏、高效氯氰菊酯、2,4-滴、烯酰吗啉具有良好的降解能力,降解率分别达到100%、63.64%、75.00%、69.23%和83.54%;经分子鉴定,NBL-B0078为枯草芽孢杆菌斯氏亚种(Bacillus subtilis subsp.spizizenii).该降解菌株能够显著降低土壤和萝卜中农药残留量,是一株具有良好开发前景的农药降解菌.

Abstract

A pesticide degrading strain NBL-B0078 was isolated from soil and water contaminated with pesticides by enrichment domestication,isolation and purification.It was identified by morphological,physio-biochemical,16S rRNA sequence analysis.And the safety of NBL-B0078 to crops and its abilities to degrade pesticide residues in soil and radish were investigated.The rusults showed that strain NBL-B0078 had good degradation ability,the degradation rates to chlorothalonil,dichlorvos,beta-cypermethrin,2,4-D and dimethomorph were 100%,63.64%,75.00%,69.23%and 83.54%,respectively.After molecular identification,strain NBL-B0078 was identified as Bacillus subtilis subsp.spizizenii.The degrading strain could significantly reduce pesticide residues in soil and radish,and it had good development prospect.

关键词

农药降解菌/枯草芽孢杆菌斯氏亚种/农药残留/百菌清/筛选

Key words

pesticide degrading strain/Bacillus subtilis subsp.spizizenii/pesticide residue/chlorothalonil/screen

引用本文复制引用

出版年

2024
现代农药
江苏省农药协会 江苏省农药研究所股份有限公司 江苏省农药科技信息站

现代农药

CSTPCD
影响因子:0.471
ISSN:1671-5284
段落导航相关论文