Study on Proliferation and Cytotoxicity of Polymeric Fiber Carrier on L929 Cells
The effects of imported polymeric fiber carriers(1#)and domestic polymeric fiber carriers(2#and 3#)on the proliferation and toxicity of L929 cells were studied.The relative proliferation rate of L929 cells in polymeric fiber carrier was detected by CCK-8 method at different extract concentration and culture time.The cytotoxicity of the material was evaluated according to general criteria,and the cell adhesion on the surface and inside of the carrier was characterized.The results show that there is no potentia cytotoxicity in groups 1#,2#and 3#when the mass fraction of the extract was between 6.25%and 100.00%,and the cytotoxicity of groups 1#,2#and 3#are graded as"1""0"and"1",respectively.In vitro cell culture test shows that the cell proliferation rate of group 2#and group 3#is similar to that of group 1#within 24 h.The cell proliferation rate of group 2#and group 3#is slightly lower than that of group 1#within 48～72 h.The cell adhesion test shows that the cell proliferation rate of group 2#and group 3#is higher than that of group 1#,and the cells tend to grow in the interior of the fiber carrier.The biocompatibility,cytotoxicity and cell adhesion of the domestic polymeric fiber carrier are all satisfactory,and the cell proliferation and cell adhesion of the 3#polymeric fiber carrier are the best.
NETL
NSTL
万方数据
