Detection of retinoic acid in mice brain tissue by LC-MS/MS
Objective The aim of this study was to establish a liquid chromatography-tandem mass spectrum (LC-MS/MS) methodology for detecting retinoic acid in mice brain tissue.Methods Multiple-reaction-monitoring (MRM) mode was utilized in electrospray ionization.The positive electric spray ionization mode was adopted.The ions for quantitative analysis of retinoic acid were 301.1→123.1.Agilent SB-C18 (100 × 2.1mm,1.8 μ m) column was adopted and the mobile phase was aqueous solution of acetonitrile and 0.1％ formic acid.Results The separation of retinoic acid from other components was good.The calibration curves were linear between 0.92-9.20 ng/g (R 2=0.9937).The average recovery rates were 93.5％,96.7％,and 103.1％ respectively,while RSD were 8.7％,6.5％,and 5.9％ respectively (n=3).Conclusion Retinoic acid was able to be detected in ten minutes and endogenous substances did not interfere with the seperation.This methodology was suitable for the detection of retinoic acid in mice brain tissue.The methodology was quick,accurate with high specificity.
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万方数据
维普
