Objective To examine the human safety and antihyperlipidemic effects of deep-sea fish oil.Methods One hundred and twenty qualified volunteers were randomly divided into testing and control groups according to the total cholesterol (TC) levels in the serum.The volunteers in the testing group were given deep-sea fish oil and the others in the control group were given placebos for 60 days.At the end of the experiment,physiologic and biochemical index of volunteers were examined,including TC,triglyceride (TG),high density lipoprotein-cholesterin (HDL-C) and low density lipoprotein-cholesterin (LDL-C) levels in the serum.Results The TC,TG and LDL-C levels of the testing group decreased significantly after given deep-sea fish oil (P<0.01); There were no significant differences between the testing and control groups in physiologic and biochemical index during the test except blood lipid.Conclusion Deep-sea fish oil is safe for human and has antihyperlipidemic effect in the current study.