Objective To establish an HPLC method for simultaneous determination of benzoyl aconitine and 6-gingerol in Sini decoction and compare the content differences of active ingredients in Sini decoction prepared by three extraction methods.Methods The detection method of the benzoyl aconitine was conducted on an C18 (4.6 mm× 250 mm,5un) column with acetonitrile-0.1 mol.L-1 ammonium acetate solution as the mobile phase using gradient elution.The flow rate was set at 1.0 mL·min-1 and detection wavelength was set at 235 nm.The column temperature was at 30 ℃.The detection method of 6-gingerol was conducted on an C18 (4.6 mm× 250 mm,5 μm) column with acetonitrile-methanol -water (40:5:55) as the mobile phase using isocratic elution.The flow rate was set at 1.0mL·min1 and the detection wavelength was set at 280 nm.The column temperature was at 25 ℃.Results The extraction rate ofbenzoyl aconitine by different methods decreased in the follow order:the combination of active ingrediets > pharmacopoeia method >traditional method.The extraction rate of 6-gingerol decreased in the follow order:traditional method ＞pharmacopoeia method ＞ the combination of active ingredients.Conclusion The established method is simple,accurate and can be used for providing a scientific basis for the improvement of extraction method of Sini decoction.
NETL
NSTL
万方数据
维普
