首页|p16INK4a联合人乳头瘤病毒及液基细胞学检查在宫颈上皮内瘤变临床诊断中的应用

p16INK4a联合人乳头瘤病毒及液基细胞学检查在宫颈上皮内瘤变临床诊断中的应用

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原文链接
  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
目的 分析p16INK4a蛋白、人乳头瘤病毒及液基细胞学检查(LCT)在宫颈癌前病变筛查中的检测效率，为宫颈癌的预防和治疗提供依据。方法 分析2019 年 1 月—2020 年6 月广州市妇女儿童医疗中心的 139 例住院患者的宫颈上皮细胞p16INK4a染色、人乳头瘤病毒检测及宫颈细胞学检测结果。这些病例包括 111 例宫颈上皮内瘤变(CIN)患者和28 例宫颈炎性疾病患者。比较三种方法单独及联合应用筛查CIN病变的效能。结果 在对CIN患者的检测中，p16INK4a、生物微流控芯片和宫颈细胞学检测CIN及以上病变的灵敏度分别为 91。89%、94。59%和 78。20%，特异度分别为 57。14%、17。86%和 46。43%，AUC 分别为 0。75、0。56 和 0。66。而"p16INK4a+LCT"、"p16INK4a+hrHPV"、"LCT+hrHPV"以及三种方法联合检测的灵敏度分别为 96。40%、97。30%、94。59%和 99。10%，特异度分别为 85。71%、92。86%、89。29%和92。86%，AUC分别为 0。91、0。95、0。92 和 0。96。结论 p16INK4a和hrHPV联合检测有助于提高诊断的准确性和早期发现率，从而使得能更早进行干预或治疗。通过这种联合应用，可以更准确地鉴别出低级别和高级别的宫颈上皮内瘤变，为个性化的病患管理提供更多信息。
Application of p16INK4a combined with human papillomavirus and liquid-based cytology in clinical diagnosis of cervical intraepithelial neoplasia
Objective To analyze the detection efficiency of p16INK4a protein combined with human papillomavirus and liquid-based cytology(LCT)in the screening of cervical precancerous lesions,and to provide a basis for cervical cancer preven-tion and treatment.Methods The results of p16INK4a staining of cervical epithelial cells,human papillomavirus testing and cer-vical cytology were analyzed in 139 inpatients at Guangzhou Women's and Children's Medical Center between January 2019 and December 2020.Of them,there were 111 patients with cervical intraepithelial neoplasia(CIN)and 28 cases of cervical inflam-matory disease.The efficacy of the three methods alone and in combination to screen for CIN lesions was compared.Results In the detection of CIN patients,the sensitivity of p16INK4a,microfluidic microarray and cervical cytology for detecting CIN and a-bove lesions was 91.89% ,94.59% and82.88% ,with specificity of 57.14% ,17.86% and46.43% ,and AUC of 0.75,0.56 and 0.65,respectively;while the sensitivity of"p16INK4a+LCT","p16INK4a+hrHPV","LCT+hrHPV"and their sen-sitivity were 96.40% ,97.30% ,94.59% and 99.10% ,their specificity was 85.71% ,92.86% ,89.29% and 92.86% ,and the AUC was 0.91,0.95,0.92 and 0.96,respectively.Conclusion The combined p16INK4a and hrHPV test helps to improve diagnostic accuracy and early detection,thus allowing for earlier intervention or treatment.This combined application allows for more accurate identification of low-grade and high-grade cervical intraepithelial neoplasia,providing more information for indi-vidualized patient management.

Human Papilloma VirusMicrofluidic Biochipsp16Ink4aLiquid-Based CytologyCervical Intraepithe-lial Neoplasia

肖雪、王宇琦、景尉、钟绍涛、赵翠柳、刘丹、谭荣韶、赖晓岚

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暨南大学附属广州红十字会医院 广东广州 510220

广州市越秀区妇幼保健院 广东广州 510060

人乳头瘤病毒 生物微流控芯片 p16INK4a 液基细胞学 宫颈上皮内瘤变

中国营养学会青年人才项目广州市卫生和计划生育科技项目广州市卫生和计划生育科技项目广州市卫生和计划生育科技项目

2020-5120211A01001720181A01001520221A040001

2024

10.3969/j.issn.1671-332X.2024.01.037

现代医院
广东省医院协会

现代医院

影响因子：1.332
ISSN：1671-332X
年,卷(期)：2024.24(1)
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