EphrinB2-mediated CDK5/ISL1 pathway enhances cardiac lymphangiogenesis and alleviates ischemic injury by resolving post-MI inflammation

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外文摘要：EphrinB2(erythropoietin-producing hepatoma interactor B2)is a key Eph/ephrin family member,promoting angiogenesis,vasculogenesis,and lymphangiogenesis during embryonic development.However,the role of EphrinB2 in cardiac lymphangiogenesis following myocardial infarction(MI)and the potential molecular mechanism remains to be demonstrated.This study revealed that EphrinB2 prevented ischemic heart post-MI from remodeling and dysfunction by activating the cardiac lymphangiogenesis signaling pathway.Deletion of EphrinB2 impaired cardiac lymphangiogenesis and aggravated adverse cardiac remodeling and ventricular dysfunction post-MI.At the same time,overexpression of EphrinB2 stimulated cardiac lymphangiogenesis which facilitated cardiac infiltrating macrophage drainage and reduced inflammation in the ischemic heart.The beneficial effects of EphrinB2 on improving clearance of inflammatory response and cardiac function were abolished in Lyve1 knockout mice.Mechanistically,EphrinB2 accelerated cell cycling and lymphatic endothelial cell proliferation and migration by activating CDK5 and CDK5-dependent ISL1 nuclear translocation.EphrinB2 enhanced the transcriptional activity of ISL1 at the VEGFR3(FLT4)promoter,and VEGFR3 inhibitor MAZ51 significantly diminished the EphrinB2-mediated lymphangiogenesis and deteriorated the ischemic cardiac function.We uncovered a novel mechanism of EphrinB2-driven cardiac lymphangiogenesis in improving myocardial remodeling and function after MI.

作者：

Yingnan Bai、Liming Chen、Fanghao Guo、Jinghong Zhang、Jinlin Hu、Xuefei Tao、Qing Lu、Wenyi Li、Xueying Chen、Ting Gong、Nan Qiu、Yawei Jin、Lifan Yang、Yu Lei、Chengchao Ruan、Qing Jing、John P.Cooke、Shijun Wang、Yunzeng Zou、Junbo Ge

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作者单位：

Department of Cardiology,Shanghai Institute of Cardiovascular Diseases,Zhongshan Hospital and Institute of Biomedical Sciences,Fudan University,Shanghai,China

Center for Reproductive Medicine & Fertility Preservation Program,International Peace Maternity and Child Health Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai,China

CAS Key Laboratory of Tissue Microenvironment and Tumor,Shanghai Institute of Nutrition and Health,Innovation Center for Intervention of Chronic Disease and Promotion of Health,University of Chinese Academy of Sciences,Chinese Academy of Sciences,Shanghai,China

Department of Cardiovascular Surgery,Guangdong Provincial Hospital of Chinese Medicine,The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,The Second Clinical College of Guangzhou University of Chinese Medicine,Guangzhou,Guangdong,China

Department of Geriatric Cardiology,Sichuan Provincial People's Hospital,University of Electronic Science and Technology of China,Chengdu,Sichuan,China

Department of Radiology,Shanghai Dongfang Hospital,Shanghai Tongji University School of Medicine,Shanghai,China

Department of Endocrinology,Tongren Hospital,Shanghai JiaoMo Tong University School of Medicine,Shanghai,China

Department of Physiology and Pathophysiology,Shanghai Key Laboratory of Bioactive Small Molecules,State Key Laboratory of Medical Neurobiology,School of Basic Medical Sciences,Fudan University,Shanghai,China

Department of Cardiovascular Sciences,Houston Methodist Research Institute,6670 Bertner Ave.,Houston,TX,USA

Minhang Hospital,Fudan University,Shanghai,China

Institute of Advanced Medicine,Henan University,Kaifeng,Henan,China

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出版年：

2024

DOI：

10.1038/s41392-024-02019-4

信号转导与靶向治疗(英文)

CSTPCD

ISSN：

年,卷(期)：2024.9(12)