Analysis of the potential mechanism underlying the unfavorable prognosis of colon cancer associated with elevated fibroblast activation protein expression
Analysis of the potential mechanism underlying the unfavorable prognosis of colon cancer associated with elevated fibroblast activation protein expression
Objective The objective of this study is to investigate the expression pattern of the fibroblast activation protein(FAP)gene in colon cancer and assess its association with patients'clinical characteristics and prognosis,aiming to provide novel insights into the pathogenesis,diagnosis,treatment,and prognosis of colon cancer.Method The RNA sequencing data of colon cancer tissues and adjacent normal tissues were downloaded from the gene expression omnibus database and the cancer gene atlas database.The expression level of FAP gene in adjacent normal tissues and colon cancer tissues was analyzed.Survival analysis was performed using colon cancer patients'survival data from Kaplan-Meier plotter database to examine the relationship between FAP gene expression and overall survival rate.Furthermore,GEPIA2 database was utilized to assess FAP gene expression in colon cancer and its prognostic predictive effect.The clinical data of colon cancer were downloaded from the gene expression omnibus database and the cancer gene atlas database to explore the expression pattern of FAP gene in different stages.To investigate molecular pathways associated with the occurrence and development of colon cancer related to FAP gene,gene enrichment analysis was conducted.Immuno-correlation analysis(CIBERSORT)was employed to explore the association between FAP gene and immune cell infiltration and immune checkpoint expression.Result Compared to adjacent normal tissues,the expression of FAP gene was upregulated in colon cancer tissues(P＜0.05).The expression level of FAPgene was different among different stages in colon cancer patients(P＜0.05).Survival analysis results demonstrated that patients with high FAP gene expression had a lower overall survival rate than those with low FAPgene expression(P＜0.05).Furthermore,FAP gene influences the occurrence and progression of colon cancer through various pathways.In colon cancer specifically,FAP gene had a negative correlation with the infiltration levels of CD4+memory T cells and plasma cells,and a positive correlation with the expression levels of common immune checkpoints.Conclusion The expression of FAP gene is upregulated in colon cancer and is strongly associated with unfavorable survival outcomes and prognosis.FAP gene plays a crucial role in promoting tumor progression through diverse cellular functions,chemical pathways,and immune infiltration,thereby establishing itself as a promising therapeutic target for the treatment of colon cancer.
维普
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