胃腺癌中Siglec7基因表达与预后及免疫微环境的关系
Relationship between Siglec7 gene expression,prognosis,and immune microenvironment in gastric adenocarcinoma
钟剑锋 1姜德培 1文学 1陈泓磊 1刘伟1
作者信息
- 1. 中山大学附属第八医院(深圳福田)消化内镜中心,广东 深圳 518033
- 折叠
摘要
目的 探讨Siglec7在胃腺癌中的表达模式及其对预后和免疫微环境的影响,并评估其作为治疗靶点的可能性.方法 从癌症基因组图谱和基因表达综合数据库中获取胃腺癌患者的RNA测序数据和临床信息,分析Siglec7及其家族基因在胃腺癌中的表达水平.通过Kaplan-Meier生存分析,评估Siglec7的表达与患者总生存期的关系.利用Pearson相关系数筛选Siglec7的共表达基因,并进行基因本体和京都基因与基因组百科全书富集分析.基于Siglec7相关基因进行胃腺癌亚型的共识聚类,将胃腺癌分为C1和C2两个亚型,并比较不同亚型的Siglec7表达水平、临床特征、生存结局和免疫微环境差异.结果 Siglec7在胃腺癌中的表达水平升高,Siglec7高表达的胃腺癌患者的总生存期较短.共表达基因分析显示,Siglec7相关基因显著富集于免疫应答调控信号通路、单核细胞分化和受体配体活性等通路.与C2亚型相比,C1亚型表现出更高的Siglec7表达、更差的临床特征和生存结局、更复杂的免疫微环境.结论 Siglec7在胃腺癌中高表达,与患者不良预后密切相关并且可能在肿瘤免疫逃逸中发挥重要作用.Siglec7不仅是胃腺癌的重要生物标志物,也是一个潜在的治疗靶点.
Abstract
Objective To investigate the expression of Siglec7 in gastric adenocarcinoma and its impact on prognosis and the immune microenvironment,and to evaluate its feasibility as a therapeutic target.Method RNA sequencing data and clinical information of gastric adenocarcinoma patients were obtained from the cancer genome atlas and gene expression omnibus databases.The expression levels of Siglec7 and its family genes in gastric adenocarcinoma were analyzed.Kaplan-Meier survival analysis was used to evaluate the relationship between Siglec7 expression and overall survival time.Pearson correlation coefficient was used to screen Siglec7 co-expressed genes,followed by gene ontology and Kyoto encyclopedia of genes and genomes enrichment analysis.Consensus clustering of gastric adenocarcinoma subtypes based on Siglec7-related genes was performed,with gastric adenocarcinoma divided into two subtypes C1 and C2,and the differences in Siglec7 expression level,clinical characteristics,survival outcomes and immune microenvironment between two subtypes were compared.Result Siglec7 expression levels were elevated in gastric adenocarcinoma,and patients with higher Siglec7 expression had shorter overall survival time.Co-expression gene analysis showed that Siglec 7-related genes were significantly enriched in immune response regulatory signaling pathways,monocyte differentiation,and receptor-ligand activity pathways.Compared to C2 subtype,C1 subtype exhibited higher Siglec7 expression,poorer clinical characteristics and survival outcomes,and a more complex immune microenvironment.Conclusion Siglec7 is highly expressed in gastric adenocarcinoma and is closely associated with poor prognosis,and may play an important role in tumor immune evasion.Siglec7 is not only an important biomarker for gastric adenocarcinoma but also a potential therapeutic target.
关键词
胃腺癌/Siglec7/免疫微环境/生物信息学分析/免疫逃逸/预后Key words
Gastric adenocarcinoma/Siglec7/Immune microenvironment/Bioinformatics analysis/Immune evasion/Prognosis引用本文复制引用
出版年
2024
NETL
NSTL
万方数据