To isolate resistance gene analogs(RCA) of melon, degenerate primers designed according to NBS-LRR conservative domain of resistance genes were used to amplify with melon genomic DNA. Seven RGAs were cloned and sequenced, out of which 821 and 324 were newly found in this research. All the RGAs were compared to discard the redundant sequence and incorporate the useful information. Different distribution status between CC-NBS-LRRs and TIR-NBS-LRRs were found by the homologous RGA analysis, and also relation of different RGA was found, such as MRGA1O and MRGH5 might be originated from common ancestor . Bioinformation and comparativegenomics method were used to discover the relation between RGAs and disease resistance genes . The Fom-1 gene conferring resistance to Fusarium oxysporum f.sp. melonis race 2 and the Prv conferring resistance to papaya ring spot virus were located between RGAs nbs47-3 (MRGH11) and MRGH21, so it was deduced that candidate gene MRGH8 and the Fom-1 were the same gene. while Prv might be the same with one of MRGH9 and MRGH1O.
NETL
NSTL
维普
万方数据
